Replication dynamics of the yeast genome
Replication dynamics of the yeast genome
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DOI：
10.1126/science.294.5540.115
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发表时间：
2001-10-05
期刊：
影响因子：
56.9
通讯作者：
Fangman, WL
中科院分区：
文献类型：
--
作者：
Raghuraman, MK;Winzeler, EA;Fangman, WL
利用寡核苷酸微阵列技术绘制了酿酒酵母染色体复制的详细图谱。通过将S期不同时间分离的复制和未复制的dna杂交到微阵列上，确定了基因组中数千个位点的复制次数。起始激活在整个S期连续发生，但大多数在S期中期发生。基因组中不同区域的复制叉运动速率差别很大。16条染色体的两端在它们的复制时间上是高度相关的。这种微阵列方法很容易适用于其他生物，包括人类。
Oligonucleotide microarrays were used to map the detailed topography of chromosome replication in the budding yeast Saccharomyces cerevisiae. The times of replication of thousands of sites across the genome were determined by hybridizing replicated and unreplicated DNAs, isolated at different times in S phase, to the microarrays. Origin activations take place continuously throughout S phase but with most firings near mid-S phase. Rates of replication fork movement vary greatly from region to region in the genome. The two ends of each of the 16 chromosomes are highly correlated in their times of replication. This microarray approach is readily applicable to other organisms, including humans.