Nanosensing of ATP by fluorescence recovery after surface energy transfer between rhodamine B and curcubit[7]uril-capped gold nanoparticles
Nanosensing of ATP by fluorescence recovery after surface energy transfer between rhodamine B and curcubit[7]uril-capped gold nanoparticles
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DOI：
10.1007/s00604-018-2901-8
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发表时间：
2018-07
影响因子：
5.7
通讯作者：
R. Kurdi;D. Patra
中科院分区：
文献类型：
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作者：
R. Kurdi;D. Patra
作者描述了一种用超分子主体分子仙茅[7]脲（CB[7]）功能化金纳米颗粒（AuNPs）的方法，仙茅[7]脲（CB[7]）可以结合罗丹明B（Rh B）。通过表面能量转移，RhB的荧光被AuNPs猝灭。加入ATP后，形成二聚体RhB-ATP复合物，并将RhB推出CB[7]。因此，荧光增加了8倍。这种荧光恢复效应已被用于开发一种新的ATP检测方案。该测定法分别在500 nm和574 nm的荧光激发和发射波长下测量，在0.5-10 μM浓度范围内工作，检测限为100 nM。该方法不受UTP、GTP、CTP、TTP、抗坏血酸和谷胱甘肽的干扰。Graphical abstractionSchematic of a method for determination of ATP in the 500 nM to 10 μM concentration range by using fluorescence recovery after surface energy transfer（SET）between rhodamine B（RhB）and gold nanoparticles capped with curbubit [7]uril（CB[7]）.
The authors describe a method for functionalization of gold nanoparticles (AuNPs) with the supramolecular host molecule, curcubit[7]uril (CB[7]) which can bind rhodamine B (RhB). The fluorescence of RhB is quenched by the AuNPs via surface energy transfer. On addition of ATP, a dimeric RhB-ATP complex is formed and RhB is pushed out of CB[7]. Hence, fluorescence increases by a factor of 8. This fluorescence recovery effect has been utilized to develop a new detection scheme for ATP. The assay, measured at fluorescence excitation and emission wavelengths of 500 nm and 574 nm respectively, works in the 0.5–10 μM concentration range and has a 100 nM detection limit. The method is not interfered by UTP, GTP, CTP, TTP, ascorbic acid and glutathione.Graphical abstractSchematic of a method for determination of ATP in the 500 nM to 10 μM concentration range by using fluorescence recovery after surface energy transfer (SET) between rhodamine B (RhB) and gold nanoparticles capped with curcubit[7]uril (CB[7]).