Immunogenicity of Neisseria gonorrhoeae lipooligosaccharide epitope 2C7, widely expressed in vivo with no immunochemical similarity to human glycosphingolipids
Immunogenicity of Neisseria gonorrhoeae lipooligosaccharide epitope 2C7, widely expressed in vivo with no immunochemical similarity to human glycosphingolipids
复制标题
DOI：
10.1093/infdis/174.6.1223
复制
发表时间：
1996-12-01
影响因子：
6.4
通讯作者：
Rice, PA
中科院分区：
文献类型：
--
作者：
Gulati, S;McQuillen, DP;Rice, PA
自然感染淋病奈瑟菌可引起针对淋球菌脂寡糖的大量抗体反应。单克隆抗体（MAb） 2C7识别了一个淋球菌脂寡糖表位，在68个连续培养阳性的生殖器分泌物中直接识别了94%的表位，在101个随机选择的新鲜淋球菌（第二代）分离物中识别了95%的表位。该表位在多次体外传代后稳定维持，不与任何已知的交叉反应性人鞘糖脂结构竞争。单抗2C7介导1个血清敏感（唾液化或不唾液化）和1个稳定血清耐药的表达表位的淋球菌分离物的人多形核白细胞体外杀伤和吞噬。淋球菌性子宫内膜炎和播散性感染引起抗2c7表位抗体升高（IgM升高6.5倍，IgG升高4.4倍，IgM升高18倍，IgG升高17倍）。淋球菌外膜疫苗免疫可使28名受试者中20人的IgG抗2c7表位抗体平均增加44.5倍。MAb 2C7鉴定的表位可能是潜在保护性淋球菌候选疫苗的极好靶点。
Natural infection with Neisseria gonorrhoeae may elicit a substantial antibody response directed against gonococcal lipooligosaccharide. Monoclonal antibody (MAb) 2C7 recognized a gonococcal lipooligosaccharide epitope, identified the epitope directly in 94% of 68 consecutive culture-positive genital secretions, and recognized 95% of 101 randomly chosen fresh (second-passage) gonococcal isolates, The epitope was stably maintained after multiple in vitro passages and did not compete with any of the known cross-reactive human glycosphingolipid structures, MAb 2C7 mediated in vitro killing and phagocytosis by human polymorphonuclear leukocytes of 1 serum-sensitive (sialylated or not) and 1 stably serum-resistant gonococcal isolate that expressed the epitope. Gonococcal endometritis and disseminated infection elicited increases (6.5-fold IgM, 4.4-fold IgG; 18-fold IgM, 17-fold IgG, respectively) in anti-2C7 epitope antibody. Immunization with a gonococcal outer membrane vaccine elicited a mean 44.5-fold increase in IgG anti-2C7 epitope antibody in 20 of 28 subjects. The epitope identified by MAb 2C7 may represent an excellent target for a potentially protective gonococcal vaccine candidate.