ULTRASTRUCTURAL CHANGES OF THE PANCREAS AND LIVER IN CYSTIC FIBROSIS.
ULTRASTRUCTURAL CHANGES OF THE PANCREAS AND LIVER IN CYSTIC FIBROSIS.
复制标题
囊性纤维化中胰腺和肝脏的超微结构变化。
DOI：
10.1093/ajcp/42.5.451
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发表时间：
1964
影响因子：
3.5
通讯作者：
P. Patterson
中科院分区：
文献类型：
--
作者：
E. Porta;A. Stein;P. Patterson
材料和方法对3、5、6、11个月和1例3周大的临床确诊为胰腺囊性纤维化症的儿童进行手术探查，以寻找胰管和肝外胆管的解剖梗阻。其他地方也报道了使用的手术程序。20探查时，取胰腺和肝脏组织进行活检，取出胆囊壁。光镜下，部分活检标本用3：1醋酸-甲醇固定，石蜡包埋，切片用苏木精-伊红、粘液、珍珠铁染、高碘酸-希夫、Gomori和Verhoff-Van Gieson染色。在电子显微镜下，取胰腺和肝脏的小切片，立即用Dalton‘s Osmic Tetroxide溶液固定1小时。在4℃下，在8：1的甲基丙烯酸丁酯与过氧化苯甲酰作为催化剂的预聚混合物中或在EPON 812中进行包埋。13个切片用Porter-Blum切片机、玻璃刀切开，在RCA型EMU-3F型电子显微镜下观察。一些超薄切片用氢氧化铅、醋酸铀酰和磷钼酸染色。21只行光镜检查。作为新鲜组织光镜研究的对照，我们复习了7例胰腺囊性纤维化和15例其他疾病的尸检资料，但肝和胰腺正常。与上述相同的染色工艺
MATERIALS AND METHODSFour children, ages 3, 5, 6, and 11 months, and 1 at 3 weeks of age, with clinically proved cystic fibrosis of the pancreas were surgically explored, in an effort to seek anatomic obstruction in pancreatic ducts and extrahepatic biliary tree. The operative procedures used were reported elsewhere. 20 At exploration, tissue was removed from the pancreas and liver for biopsies, and the gallbladder was removed.For light microscopy, portions of the biopsy specimens were fixed in 3: 1 acetic acid-methyl alcohol, embedded in paraffin, and sections stained by means of hematoxylin-eosin, mucicarmine, Pearl's iron stain, periodic acid-Schiff, Gomori, and Verhoff-Van Gieson stain technics. For electron microscopy small sections of tissue from pancreas and liver were immediately fixed in Dalton's osmic tetroxide solution7 for 1 hr. at 4 C. Embedding was performed either in a prepolymerized mixture of 8: 1 butyl-methyl methacrylate with the addition of benzoyl peroxide as a catalyst, or in Epon 812. 13 Sections were cut with a Porter-Blum microtome, using glass knives, and examined with an RCA model EMU-3F electron microscope. Some of the ultrathin sections were stained with lead hydroxide, uranyl acetate, and phosphomolybdic acid. 21 The gallbladders were examined only with light microscopy. As control for light microscopic studies of the fresh tissues, autopsy material from 7 patients with cystic fibrosis of the pancreas and 15 children with other diseases, but normal livers and pancreases, were reviewed. The same stain technics as described above