Cellular approach to cryopreservation of embryos
Cellular approach to cryopreservation of embryos
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DOI：
10.1016/0093-691x(95)00351-8
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发表时间：
1996-01-01
期刊：
影响因子：
2.8
通讯作者：
Dobrinsky, JR
中科院分区：
文献类型：
--
作者：
Dobrinsky, JR
在改进和简化胚胎移植程序中常规使用的冷冻保存程序方面已经取得了相当大的进展。传统的慢速、可编程冷冻和玻璃化胚胎为兽医、科学家和生产者提供了牛群繁殖实践的替代方案，然而，冷冻保存后的妊娠率并不等同于新鲜胚胎移植。无论胚胎如何冷冻保存，总会有一些死亡，这对生产者来说是昂贵的。记录冷冻保存期间或之后的细胞损伤将为理解细胞对冷冻保存的敏感性提供有用的非经验信息，并将导致胚胎冷冻保存的改进方案和更好地理解家畜胚胎学。本文讨论了目前在胚胎冷冻保存的进展，并将解决细胞内损伤冷冻胚胎的细胞结构，并试图绕过这种损害，利用细胞骨架稳定冷冻前。
Considerable progress has been made in the improvement and simplification of cryopreservation procedures routinely used in embryo transfer programs. Conventional slow-rate, programmable freezing and vitrification of embryos have given veterinarians, scientists and producers alternatives in their herd reproduction practices, however, pregnancy rates after cryopreservation are not equivalent to fresh embryo transfer. No matter how embryos are cryopreserved, some always die, and that can be costly to the producer. Documenting cellular damage during or after cryopreservation would provide useful, non-empirical information for understanding cellular sensitivities to cryopreservation and will lead to improved protocols for embryo cryopreservation and a better understanding of domestic animal embryology. This paper discusses the current progress in embryo cryopreservation, and will address intracellular damage to the cytoarchitecture of cryopreserved embryos and attempts to detour this damage by utilizing cytoskeletal stabilization prior to cryopreservation.