Centrophobism/thigmotaxis, a new role for the mushroom bodies in Drosophila
Centrophobism/thigmotaxis, a new role for the mushroom bodies in Drosophila
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DOI：
10.1002/neu.20111
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发表时间：
2005-02-15
期刊：
影响因子：
--
通讯作者：
Martin, JR
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文献类型：
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作者：
Besson, M;Martin, JR
昆虫，像脊椎动物一样，在觅食或导航时表现出空间复杂的运动活动模式。最近的野外研究表明，果蝇会避开中心区域，停留在外围区域，这种效应可以解释为中心恐惧症和/或趋近性。在这项研究中，我们进一步表征了这一现象，并研究了潜在的神经机制。果蝇蘑菇体（MBs）在嗅觉学习和记忆过程中的意义已经得到了很好的证明。在开阔的野外环境中，通过视频跟踪记录了苍蝇的运动活动，我们发现羟基脲消融的MBs大大减少了苍蝇的中心回避，这表明这些结构具有新的作用。此外，动力蛋白基因shibire的温度敏感等位基因在各种增强陷阱P[GAL4]系中表达，破坏了不同NIB叶的突触传递。特异性阻断γ叶可改变嗜心/吸心性，而阻断α / β叶则不会，提示NIB叶的功能专门化。果蝇可以作为一种新的模型系统，用于阐明嗜心/嗜心等复杂现象的遗传和神经基础。(C) Wiley期刊公司
Insects, like vertebrates, exhibit spatially complex locomotor activity patterns when foraging or navigating. Open field studies recently showed that Drosophila avoids central zones and stays at the periphery, an effect that can be interpreted as centrophobism and/or thigmotaxis. In this study, we further characterized this phenomenon and studied the responsible underlying neural mechanisms. The implication of the Drosophila mushroom bodies (MBs) in olfactory learning and memory processes is well documented. In an open field situation in which fly locomotor activity is recorded by video tracking, we show that center avoidance is greatly diminished in flies with hydroxyurea-ablated MBs, suggesting a new role for these structures. Furthermore, the temperature-sensitive allele of the dynamin gene shibire was expressed in various enhancertrap P[GAL4] lines, disrupting synaptic transmission in different NIB lobes. Specifically blocking the gamma lobes alters centrophobism/thigmotaxis while blocking the alpha/beta lobes does not, suggesting a functional specialization of NIB lobes. Drosophila may serve as a new model system for elucidating the genetic and neural bases of such complex phenomena as centrophobism/thigmotaxis. (C) Wiley Periodocals, Inc.