Antioxidant Capacity of Pure Compounds and Complex Mixtures Evaluated by the ORAC-Pyrogallol Red Assay in the Presence of Triton X-100 Micelles
Antioxidant Capacity of Pure Compounds and Complex Mixtures Evaluated by the ORAC-Pyrogallol Red Assay in the Presence of Triton X-100 Micelles
复制标题
在 Triton X-100 胶束存在下通过 ORAC-连苯三酚红测定评估纯化合物和复杂混合物的抗氧化能力
作者：
M. Romero;B. Rojano;J. Mella;C. Pessoa;E. Lissi;C. López
研究了在Triton X-100胶束存在和不存在的情况下，不同抗氧化剂及其复配物对过氧自由基诱导的邻苯三酚红（PGR）消耗的保护作用。胶束的存在显著降低了亲脂性抗氧化剂（β-胡萝卜素、没食子酸辛酯）提供的对PGR的保护，而对于亲水性抗氧化剂如Trolox、咖啡酸、没食子酸和抗坏血酸没有观察到胶束的影响。在复杂的混合物的存在下，Triton X-100胶束的明显效果也观察到在葡萄酒，茶浸液，和种子提取物的Eugenia jambolana和Myrciaria cauliflora的保护。另一方面，没有观察到胶束对橙子汁和果肉提取物的影响。在不存在（ORAC-PCR）和存在Triton X-100胶束（ORAC-PGRMIC）的情况下评价ORAC（氧自由基吸收能力）指数。Triton X-100胶束以亲脂性依赖的方式影响抗氧化剂的ORAC-PGR值。从所获得的结果，我们得出结论，ORAC-PCR和ORAC-PGRMIC测定可以被认为是一种替代方法，以估计抗氧化能力（ORAC-PCR），并推断关联到Triton X-100胶束（ORAC-PCR/ORAC-PGRMIC）的纯抗氧化剂及其复杂的混合物。
The protective effect of different antioxidants and complex mixtures on the consumption of pyrogallol red (PGR) induced by peroxyl radicals was studied in the absence and presence of Triton X-100 micelles. The presence of micelles decreased significantly the protection of PGR afforded by lipophilic antioxidants (β-carotene, octyl gallate), while no effect of micelles was observed for hydrophilic antioxidants such as Trolox, caffeic acid, gallic acid, and ascorbic acid. In the presence of complex mixtures a clear effect of Triton X-100 micelles was also observed in the protection afforded by wines, tea infusions, and seed extracts of Eugenia jambolana and Myrciaria cauliflora. On the other hand, no effect of micelles was observed for orange juice and pulp fruit extracts. The ORAC (Oxygen Radical Absorbance Capacity) index was evaluated in the absence (ORAC-PGR) and presence of Triton X-100 micelles (ORAC-PGRMIC). Triton X-100 micelles affect ORAC-PGR values of antioxidants in a lipophilicity-dependent way. From the obtained results, we conclude that ORAC-PGR and ORAC-PGRMIC assays could be considered as an alternative to estimate the antioxidant ability (ORAC-PGR) and to infer the association to Triton X-100 micelles (ORAC-PGR/ORAC-PGRMIC) of pure antioxidants and their complex mixtures.