Role of hydroxyapatite nanoparticle size in bone cell proliferation
Role of hydroxyapatite nanoparticle size in bone cell proliferation
复制标题
羟基磷灰石纳米颗粒尺寸在骨细胞增殖中的作用
DOI：
10.1039/b705129h
复制
发表时间：
2007-01-01
影响因子：
--
通讯作者：
Tang, Ruikang
中科院分区：
文献类型：
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作者：
Cai, Yurong;Liu, Yukan;Tang, Ruikang
制备了直径为20 ± 5、40 ± 10和80 ± 12 nm的羟基磷灰石（HAP）纳米颗粒，并研究了其对两种骨相关细胞骨髓间充质干细胞（MSCs）和骨肉瘤细胞（U2OS）增殖的影响。细胞培养实验表明，与传统的HAP相比，纳米相矿物的嗜细胞性有所改善。在纳米HAP上测量到更大的细胞活力和MSC增殖，对于20 nm尺寸的颗粒显著。有趣的是，纳米HAP对骨肉瘤细胞的生长有抑制作用，20 nm大小的颗粒是最好的阻滞剂。结果表明，HAP纳米颗粒可以表现出良好的细胞增殖，以优化生物功能，其中颗粒大小被认为起着关键作用。这些体外研究结果对于理解纳米粒子在生物矿化过程中的亲细胞性和生物活性具有重要意义。
Hydroxyapatite (HAP) nanoparticles, typically 20 +/- 5, 40 +/- 10 and 80 +/- 12 nm in diameter, were prepared and their effects on the proliferation of two bone- related cells, bone marrow mesenchymal stem cells (MSCs) and osteosarcoma cells (U2OS) were studied. The cell culture experiments showed improved cytophilicity of the nanophase mineral as compared with conventional HAP. Greater cell viability and proliferation of MSCs were measured on the nano HAP, remarkably for 20 nm sized particles. Interestingly, the growth of osteosarcoma cells was inhibited by the nano HAP and 20 nm sized particles were the best retardant. It is suggested that the HAP nanoparticles can exhibit favorable cell proliferation to optimize biological functionality, in which the particle size is believed to play a key role. These in vitro findings are of great significance for the understanding of cytophilicity and biological activity of nanoparticles during biomineralization.