Lumbee traditional medicine: Neuroprotective activities of medicinal plants used to treat Parkinson's disease-related symptoms.
Lumbee traditional medicine: Neuroprotective activities of medicinal plants used to treat Parkinson's disease-related symptoms.
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DOI：
10.1016/j.jep.2017.02.021
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发表时间：
2017-07-12
影响因子：
5.4
通讯作者：
Rochet JC
中科院分区：
文献类型：
--
作者：
de Rus Jacquet A;Timmers M;Ma SY;Thieme A;McCabe GP;Vest JHC;Lila MA;Rochet JC
帕金森病（Parkinson's disease，PD）是一种神经退行性疾病，其特征是黑质多巴胺能神经元丢失，存活神经元中存在富含突触前蛋白α-突触核蛋白（aSyn）聚集形式的路易体包涵体。虽然目前的治疗提供了暂时的症状缓解，但它们并不能减缓中脑中潜在的神经变性。在这项研究中，我们分析了Lumbee部落成员用于治疗PD相关症状的当代草药实践，以确定治疗PD的安全有效的草药。本研究的目的是（i）记录Lumbee印第安人用于治疗PD和PD相关症状的药用植物，以及（ii）表征候选植物的子集，以减轻PD相关损伤引起的神经毒性及其潜在的神经保护机制。在北卡罗来纳州彭布罗克和周围城镇对伦比治疗师和当地人进行了采访。收集植物样品并制备为水提取物用于随后的分析。提取物的特征在于其诱导皮质星形胶质细胞中核因子E2相关因子2（Nrf 2）抗氧化反应活化的能力。进一步检查了由蓝接骨木花制备的提取物（接骨木花提取物）在诱导多能干细胞（iPSC）衍生的星形胶质细胞和原代中脑培养物中诱导Nrf 2介导的转录、改善线粒体功能障碍和减轻鱼藤酮或aSyn介导的神经毒性的能力。民族药理学访谈记录了32种用于治疗PD相关症状的药用植物和40种用于治疗其他疾病的植物。从接骨木花中制备的富含多酚的提取物激活了皮质星形胶质细胞、iPSC衍生的星形胶质细胞和原代中脑培养物中Nrf 2介导的抗氧化反应，显然是通过抑制泛素蛋白酶体系统介导的Nrf 2降解。此外，接骨木花提取物挽救了神经元细胞系中的线粒体功能缺陷，并减轻了原代中脑培养物中鱼藤酮和aSyn引起的神经毒性。这些结果突出了传统Lumbee医学中使用的植物提取物的潜在治疗益处，并且它们提供了对接骨木花提取物可以抑制由环境和遗传PD相关损伤引起的神经毒性的机制的深入了解。
Parkinson’s disease (PD) is a neurodegenerative disorder characterized by a loss of dopaminergic neurons in the substantia nigra pars compacta and the presence in surviving neurons of Lewy body inclusions enriched with aggregated forms of the presynaptic protein α-synuclein (aSyn). Although current therapies provide temporary symptomatic relief, they do not slow the underlying neurodegeneration in the midbrain. In this study, we analyzed contemporary herbal medicinal practices used by members of the Lumbee tribe to treat PD-related symptoms, in an effort to identify safe and effective herbal medicines to treat PD. The aims of this study were to (i) document medicinal plants used by Lumbee Indians to treat PD and PD-related symptoms, and (ii) characterize a subset of plant candidates in terms of their ability to alleviate neurotoxicity elicited by PD-related insults and their potential mechanisms of neuroprotection. Interviews of Lumbee healers and local people were carried out in Pembroke, North Carolina, and in surrounding towns. Plant samples were collected and prepared as water extracts for subsequent analysis. Extracts were characterized in terms of their ability to induce activation of the nuclear factor E2-related factor 2 (Nrf2) antioxidant response in cortical astrocytes. An extract prepared from Sambucus caerulea flowers (elderflower extract) was further examined for the ability to induce Nrf2-mediated transcription in induced pluripotent stem cell (iPSC)-derived astrocytes and primary midbrain cultures, to ameliorate mitochondrial dysfunction, and to alleviate rotenone- or aSyn-mediated neurotoxicity. The ethnopharmacological interviews resulted in the documentation of 32 medicinal plants used to treat PD-related symptoms and 40 plants used to treat other disorders. A polyphenol-rich extract prepared from elderflower activated the Nrf2-mediated antioxidant response in cortical astrocytes, iPSC-derived astrocytes, and primary midbrain cultures, apparently via the inhibition of Nrf2 degradation mediated by the ubiquitin proteasome system. Furthermore, the elderflower extract rescued mitochondrial functional deficits in a neuronal cell line and alleviated neurotoxicity elicited by rotenone and aSyn in primary midbrain cultures. These results highlight potential therapeutic benefits of botanical extracts used in traditional Lumbee medicine, and they provide insight into mechanisms by which an elderflower extract could suppress neurotoxicity elicited by environmental and genetic PD-related insults.
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DOI：
10.1073/pnas.0813361106
发表时间：
2009-02-24
影响因子：
11.1
作者：
Chen, Pei-Chun;Vargas, Marcelo R.;Johnson, Jeffrey A.
通讯作者：
Johnson, Jeffrey A.
DOI：
10.1080/09637480410001725148
发表时间：
2004-05-01
影响因子：
3.9
作者：
Chun, OK;Smith, N;Lee, CY
通讯作者：
Lee, CY
影响因子：
4.7
作者：
Bahia, Parmvir K.;Rattray, Marcus;Williams, Robert J.
通讯作者：
Williams, Robert J.
影响因子：
4.8
作者：
Alam, J;Stewart, D;Cook, JL
通讯作者：
Cook, JL
影响因子：
--
作者：
Colín-González AL;Santana RA;Silva-Islas CA;Chánez-Cárdenas ME;Santamaría A;Maldonado PD
通讯作者：
Maldonado PD