Identification of DNA components required for induction of cotton leaf curl disease
Identification of DNA components required for induction of cotton leaf curl disease
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DOI：
10.1006/viro.2001.0949
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发表时间：
2001-07-05
期刊：
影响因子：
3.7
通讯作者：
Markham, PG
中科院分区：
文献类型：
--
作者：
Briddon, RW;Mansoor, S;Markham, PG
棉花曲叶病（CLCuD）是巴基斯坦棉花生产的主要制约因素。单分型菜豆花叶病毒棉花曲叶病毒（CLCuV）的感染性克隆，与患病的棉花，不能在寄主植物中诱导典型的症状。我们已经确定并分离出一个单链DNA分子长度约1350个核苷酸，当coinoculated与菜豆花叶病毒对棉花，诱导典型的症状CLCuD，包括静脉肿胀，静脉变暗，叶片卷曲，和enations。这种分子（称为DNA β）需要菜豆病毒进行复制和降解。CLCuV/DNA 1/DNA β复合物与先前在胜红蓟中鉴定的类似复合物一起，代表了一种全新类型的传染性致病因子的成员。这一发现的影响，我们了解新的致病剂的演变进行了讨论。(C)北京：科学出版社.
Cotton leaf curl disease (CLCuD) is a major constraint to cotton production in Pakistan. Infectious clones of the monopartite begomovirus cotton leaf curl virus (CLCuV), associated with diseased cotton, are unable to induce typical symptoms in host plants. We have identified and isolated a single-stranded DNA molecule approximately 1350 nucleotides in length which, when coinoculated with the begomovirus to cotton, induces symptoms typical of CLCuD, including vein swelling, vein darkening, leaf curling, and enations. This molecule (termed DNA beta) requires the begomovirus for replication and encapsidation. The CLCuV/DNA 1/DNA beta complex, together with a similar complex previously identified in Ageratum conyzoides, represent members of an entirely new type of infectious, disease-causing agents. The implications of this finding to our understanding of the evolution of new disease-causing agents are discussed. (C) 2001 Academic Press.