Oriented adhesion of Escherichia coli to polystyrene particles
Oriented adhesion of Escherichia coli to polystyrene particles
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DOI：
10.1128/aem.69.11.6515-6519.2003
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发表时间：
2003-11-01
影响因子：
4.4
通讯作者：
Velegol, D
中科院分区：
文献类型：
--
作者：
Jones, JF;Feick, JD;Velegol, D
已使用多种技术观察到无鞭毛大肠杆菌 K-12 菌株对聚苯乙烯 (PS) 乳胶球或玻璃毛细管的粘附。人们的注意力集中在棒状细菌在 100 mM 磷酸盐缓冲盐水中粘附到表面时的方向。数据显示，PS颗粒90%以上的时间都粘附在细菌的末端。此外，PS 颗粒仅粘附在一端，而不是两端。同样，对于粘附在玻璃上的细菌的实验，细菌粘附在玻璃的末端。为了确定细菌末端是否具有与中部不同的电荷密度，使用了旋转电泳实验。这些实验表明没有可测量的电荷不均匀性。为了检查细菌对 PS 颗粒的粘附程度，使用了差示电泳。几乎总是发现细菌不可逆转地粘附在 PS 球上。定向粘附的原因不太可能是由于表面脂多糖 (LPS)，因为所使用的三种 K-12 菌株（每种菌株具有不同的 LPS 长度）表现出相似的行为。结果根据细菌细胞极性进行讨论。数据表明，细菌末端的纳米结构域对于粘附很重要，并且不可逆粘附的时间尺度很短。
The adhesion of nonflagellated Escherichia coli strain K-12 to polystyrene (PS) latex spheres or glass capillaries has been observed by using several techniques. Attention was focused on the orientation of the rod-shaped bacteria as they adhered to the surfaces in 100 mM phosphate-buffered saline. Data show that PS particles adhered to the ends of the bacteria more than 90% of the time. Moreover, the PS particles adhered to one end only, never to both. Similarly, for experiments with bacteria adhering to glass, the bacteria adhered on their ends. In order to determine whether the end of a bacterium had a different charge density from that of the middle, rotational electrophoresis experiments were used. These experiments indicated no measurable charge nonuniformity. In order to examine how strongly adhered the bacteria were to the PS particles, differential electrophoresis was used. Almost always, bacteria were found to be irreversibly adhered to the PS spheres. The cause of the oriented adhesion is not likely due to surface lipopolysaccharides (LPS), since the three strains of K-12 that were used, each having a different length of LPS, showed similar behavior. The results are discussed in terms of bacterial cell polarity. The data indicate that nanodomains on the bacterial ends are important for adhesion and that the time scale for irreversible adhesion is short.