Multiple roles of nitrate transport accessory protein NAR2 in plants
Multiple roles of nitrate transport accessory protein NAR2 in plants
复制标题
硝酸盐转运辅助蛋白 NAR2 在植物中的多重作用
DOI：
10.4161/psb.6.9.16377
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发表时间：
2011-01-01
影响因子：
2.9
通讯作者：
Xu, Guohua
中科院分区：
文献类型：
--
作者：
Feng, Huimin;Fan, Xiaorong;Xu, Guohua
两组分高亲和力硝酸盐转运系统NAR2/NRT2已在几种植物中被定义。在拟南芥中，AtNAR2.1具有将AtNRT2.1靶向到质膜上的作用。基因敲除突变体atnar2.1缺乏诱导性高亲和力运输系统(IHATS)活性，对初生根的侧根启动也表现出与atnrt2.1相同的抑制作用，以响应低硝酸盐供应。在水稻中，OsNAR2.1与OsNRT2.1、OsNRT2.2和OsNRT2.3a相互作用，在高浓度和低浓度范围内提供硝酸盐吸收。在水稻根中，OsNAR2.1及其伴侣NRT2s在组织特异性和丰度上表现出一定的差异。OsNAR2.1基因的敲除抑制了参与侧根发育硝酸盐信号通路的ANR1同源基因OsMADS(AK241644)的表达，并导致侧根长度减少，尽管根中存在硝酸盐状态。可以预见，NAR2除了在植物中作为iHATS的一个组成部分外，还具有多种功能。
Two component high affinity nitrate transport system, NAR2/NRT2, has been defined in several plant species. In Arabidopsis, AtNAR2.1 has a role in the targeting of AtNRT2.1 to the plasma membrane. The gene knock out mutant atnar2.1 lacks inducible high-affinity transport system (IHATS) activity, it also shows the same inhibition of lateral root (LR) initiation on the newly developed primary roots as the atnrt2.1 mutant in response to low nitrate supply. In rice, OsNAR2.1 interacts with OsNRT2.1, OsNRT2.2 and OsNRT2.3a to provide nitrate uptake over high and low concentration ranges. In rice roots OsNAR2.1 and its partner NRT2s show some expression differences in both tissue specificity and abundance. Knockdown of OsNAR2.1 suppressed expression of OsMADS (AK241644), a homologous gene of ANR1 involved in the nitrate signaling pathway of lateral root development and resulted in decreased lateral root length despite of nitrate status in the roots. It can be predicted that NAR2 plays multiple roles in addition to being an IHATS component in plants.