Enhancement of Optical Transmission Capacity of Isolated Structures in the Brain of Mature Mice
Enhancement of Optical Transmission Capacity of Isolated Structures in the Brain of Mature Mice
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DOI：
10.1007/s10517-009-0463-9
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发表时间：
2009-01-01
影响因子：
0.7
通讯作者：
Anokhin, K. V.
中科院分区：
文献类型：
--
作者：
Efimova, O. I.;Anokhin, K. V.
We developed an efficient method of optical clarification of isolated structures in the brain of mature mice. The method decreases optical density and opens the way to use novel methods of optical tomography in the study of the brain tissue in mature organism. Specimens dehydrated with 2-butoxyethanol, destained with Dent solution, and cleared in benzyl benzoate:benzyl alcohol or in water solution of iothalamate had the lowest optical density. The clearing effect did not directly depend on the refraction index of the composition. Possible mechanisms of increasing optical transmission of myelinated structures in the brain are discussed.
