Matrine Inhibits Mouse Sperm Function by Reducing Sperm [Ca2+]i and Phospho-ERK1/2
Matrine Inhibits Mouse Sperm Function by Reducing Sperm [Ca2+]i and Phospho-ERK1/2
复制标题
苦参碱通过减少精子 [Ca2 ]i 和 Phospho-ERK1/2 抑制小鼠精子功能
DOI：
10.1159/000369703
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发表时间：
2015-01-01
影响因子：
--
通讯作者：
Zeng, Xu-hui
中科院分区：
文献类型：
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作者：
Luo, Tao;Zou, Qian-xing;Zeng, Xu-hui
背景：苦参碱是一种生物活性生物碱，具有多种药理作用，在中药中应用广泛。然而，它对男性生殖的影响并不清楚。本研究旨在探讨苦参碱对小鼠成熟精子的体外毒性。方法：体外培养小鼠附睾尾精子，用苦参碱（10-200 μM）处理。观察小鼠精子的活力、运动力、获能、顶体反应和受精能力。此外，还分析了精子的细胞内钙离子浓度（[Ca 2 +]i）、钙（Cath）和钾（K）电流以及细胞外信号调节激酶1/2（p-ERK 1/2）的磷酸化。结果如下：在暴露于100 µM或更高浓度的苦参碱后，小鼠附睾尾精子表现出由Ca 2+离子载体A23187诱导的总运动力、前向运动力、线速度和顶体反应率显著降低。结果表明，苦参碱能显著降低小鼠精子的受精能力。我们的数据进一步表明，苦参碱显着降低精子[Ca 2 +]i和[Ca 2 +] i相关的p-ERK 1/2;然而，CatSper和KSper电流，这被认为是相互作用调节精子中的Ca 2+内流，不受苦参碱的影响。结论：苦参碱通过降低精子[Ca 2 +]i和抑制ERK 1/2磷酸化而抑制小鼠精子功能。
Background: Matrine is a bioactive alkaloid that has a variety of pharmacological effects and is widely used in Chinese medicine. However, its effects on male reproduction are not well known. In this study, we aimed to investigate the in vitro toxicity of matrine on mature mouse sperm. Methods: Mouse cauda epididymal sperm were exposed to matrine (10-200 µM) in vitro. The viability, motility, capacitation, acrosome reaction and fertilization ability of the mouse sperm were examined. Furthermore, the intracellular calcium concentration ([Ca2+]i), calcium (Catsper) and potassium (Ksper) currents, and phosphorylation of extracellular signal regulated kinases 1/2 (p-ERK1/2) of the sperm were analyzed. Results: After exposure to 100 µM or more of matrine, mouse cauda epididymal sperm exhibited a significant reduction in total motility, progressive motility, linear velocity and acrosome reaction rate induced by Ca2+ ionophore A23187. As a result, the fertilization ability of mouse sperm was remarkably decreased by matrine. Our data further demonstrated that matrine significantly reduced sperm [Ca2+]i and [Ca2+]i-related p-ERK1/2; however, both the CatSper and KSper currents, which are thought to interactively regulate Ca2+ influx in sperm, were not affected by matrine. Conclusion: Our findings indicate that matrine inhibits mouse sperm function by reducing sperm [Ca2+]i and suppressing the phosphorylation of ERK1/2.