Sox9 transactivation and testicular expression of a novel human gene, KIAA0800.
Sox9 transactivation and testicular expression of a novel human gene, KIAA0800.
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Sox9 反式激活和新型人类基因 KIAA0800 的睾丸表达。
DOI：
10.1002/jcb.10214
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Chinnadurai,G
中科院分区：
文献类型：
--
作者：
Zhao,Ling-Jun;Zhang,Shangao;Chinnadurai,G
The Sry and Sox9 sex‐determination factors initiate and promote testis differentiation by gene transactivation through similar promoter elements. However, knowledge is limited concerning what genes are regulated by Sry/Sox9 in the testis. Identification and characterization of Sry/Sox9‐regulated genes are critical for understanding sexual differentiation. We now demonstrate that a novel human gene,KIAA0800, is preferentially expressed in the testis and is transactivated by Sox9. TheKIAA0800promoter is repressed by an upstream element involving a polyT track and twoAlurepeats. Two specific Sox9‐bindings sites have been identified in theKIAA0800promoter by using DNaseI footprinting assays and gel electrophoretic mobility shift assays. Sox9 transactivation of theKIAA0800promoter appears to be exerted mainly through the relief of promoter repression. Genes homologous to the humanKIAA0800exist in organisms with differentiated sex tissues including mouse,Drosophila, andC. elegans, but not in unicellular organisms, including yeast and bacteria. Further, our recent sequence analysis shows that KIAA0800 protein is 97% identical between human and mouse. Thus,KIAA0800is a novel Sox9‐activated gene that is evolutionarily conserved and potentially involved in sexual differentiation. J. Cell. Biochem. 86: 277–289, 2002. © 2002 Wiley‐Liss, Inc.
