Comparative study of cardiac electrophysiological effects of atrial natriuretic peptide
Comparative study of cardiac electrophysiological effects of atrial natriuretic peptide
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DOI：
10.1007/bf00240031
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发表时间：
1996-07-01
影响因子：
4.3
通讯作者：
Nanasi, P
中科院分区：
文献类型：
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作者：
Kecskemeti, V;Pacher, P;Nanasi, P
在各种（人、兔、豚鼠）心房和豚鼠右心室乳头肌中研究了心房钠尿肽（ANP）对动作电位特征的影响。 ANP (1-100 nM) 不会改变静息膜电位，也不会改变去极化相的最大速率 (V-max)。 ANP 剂量依赖性地降低豚鼠心房肌和心室肌的动作电位幅度，最高可达 10 nM，但在其他心房制剂中并不影响该参数。在几乎所有研究的心脏制剂中，ANP 都会引起剂量依赖性的动作电位持续时间 (APD) 显着下降（豚鼠左心房除外）。在人类心房和豚鼠心室纤维中可以观察到对 APD 的最强影响。 K+ 通道阻滞剂 4-氨基吡啶 (1 mM) 和 ATP 依赖性 K+ 通道抑制剂格列本脲 (10 μM) 可阻止 ANP 对两种心室心房制剂中 APD 的影响。 ANP 阻止了异丙肾上腺素（0.5 μM）在 K+ 去极化心肌中诱导的慢 AP 的出现。目前的数据表明，ANP 可能会抑制缓慢的内向 Ca2+ 通道活性并促进 K+ 通道活性。
The effects of atrial natriuretic peptide (ANP) on action potential characteristics were studied in various (human, rabbit, guineapig) atrial and guinea-pig right ventricular papillary muscles. ANP (1-100 nM) did not modify the resting membrane potential nor the maximum rate of depolarization phase (V-max). Up to 10 nM, ANP dose-dependently decreased the action potential amplitude both in guinea-pig atrial and ventricular muscles, but it did not affect this parameter in the other atrial preparations. ANP caused a dose-dependent, marked decrease of action potential duration (APD) in practically every cardiac preparation studied (exception of guinea-pig left atrium). The strongest effect on APD can be observed in human atrial and guinea-pig ventricular fibers. The K+ channel blocker 4-aminopyridine (1 mM) and the ATP-dependent K+ channel inhibitor glibenclamide (10 mu M) prevented the effect of ANP on APD in both ventricular atrial preparations. ANP prevented the appearance of isoprenaline (0.5 mu M) induced slow AP in K+ depolarized myocardium. The present data suggest that ANP may inhibit the slow inward Ca2+ channel activity and facilitate the K+ channel activity.