Production of a fusion protein consisting of the enterotoxigenic Escherichia coli heat-labile toxin B subunit and a tuberculosis antigen in Arabidopsis thaliana
Production of a fusion protein consisting of the enterotoxigenic Escherichia coli heat-labile toxin B subunit and a tuberculosis antigen in Arabidopsis thaliana
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DOI：
10.1007/s00299-003-0718-2
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发表时间：
2004-02-01
影响因子：
6.2
通讯作者：
Walmsley, AM
中科院分区：
文献类型：
--
作者：
Rigano, MM;Alvarez, ML;Walmsley, AM
转基因植物是生产和粘膜递送保护性抗原的潜在安全和廉价的载体。然而，该技术的应用受到免疫系统对非颗粒亚单位疫苗的不良反应的限制。治疗性蛋白质与载体蛋白质的共递送可以增加抗原的有效性。本文报道了转基因拟南芥植物产生融合蛋白的能力，该融合蛋白由大肠杆菌不耐热肠毒素的B亚基和6 kDa的结核病抗原组成，早期分泌抗原靶标ESAT-6。融合蛋白的两种组分均用GM 1-神经节苷脂依赖的酶联免疫吸附试验检测。这表明融合蛋白保留了其天然抗原性和形成五聚体的能力。
Transgenic plants are potentially safe and inexpensive vehicles to produce and mucosally deliver protective antigens. However, the application of this technology is limited by the poor response of the immune system to non-particulate, subunit vaccines. Co-delivery of therapeutic proteins with carrier proteins could increase the effectiveness of the antigen. This paper reports the ability of transgenic Arabidopsis thaliana plants to produce a fusion protein consisting of the B subunit of the Escherichia coli heat-labile enterotoxin and a 6 kDa tuberculosis antigen, the early secretory antigenic target ESAT-6. Both components of the fusion protein were detected using GM1-ganglioside-dependent enzyme-linked immunosorbant assay. This suggested the fusion protein retained both its native antigenicity and the ability to form pentamers.