Characterization of a complementary deoxyribonucleic acid coding for the gamma chain of human fibrinogen.
Characterization of a complementary deoxyribonucleic acid coding for the gamma chain of human fibrinogen.
复制标题
编码人纤维蛋白原γ链的互补脱氧核糖核酸的表征。
DOI：
10.1021/bi00282a033
复制
发表时间：
1983
期刊：
影响因子：
2.9
通讯作者：
Davie,EW
中科院分区：
文献类型：
--
作者：
Chung,DW;Chan,WY;Davie,EW
陈伟仪，5和Earl W.Davie**摘要：利用人工合成的核苷酸混合物作为探针，从肝cDNA库中分离到了编码人纤维蛋白原7链的多个cDNA。用其中一个阳性克隆对18000个重组子的整个文库进行筛选，得到了320个阳性克隆。全长1638个碱基对，5‘端含有10个碱基对的聚(G)，其次是71个碱基对的非编码核苷酸。接下来的78个碱基对编码了一个26个氨基酸的前导序列，包括一个蛋氨酸起始信号和一个典型的疏水核心。下面的1233个碱基对编码了成熟蛋白质中存在的411个氨基酸，紧随其后的是TAA的终止密码子、207个碱基对的非编码核苷酸、15个碱基对的聚(A)轨迹和22个碱基对的聚(C)。然后将这7个链的特定区域与人纤维蛋白原的a和8链的cDNA进行比较。人纤维蛋白原E7链(Mr 46500)由411个氨基酸组成(LottSpeich&Henschen，1977；Henschen&LottSpeich，1977)和与ASN-52相连的单一碳水化合物链(Iwanaga等人，1968)。两条7链与2条a链和2条8链通过链内二硫键交联，形成含有3个结节的膨胀型多域结构。7链的内部部分(氨基酸残基24-134)由独特的胱氨酸残基结合，位于连接三个结核的域间连接结构中(Doolitte等人，1978年)。羧基-三者-
Dominic W. Chung, Wai-Yee Chan, 5 and Earl W. Davie** abstract: A number of cDNAs coding for the 7 chain of human fibrinogen havebeen isolated from a liver cDNA li-brary by employing a synthetic nucleotide mixture as a probe. One of the positive clones was then employed to screen the entire cDNA library of 18 000 recombinants, yielding 320 positive clones for the 7 chain. The largest cDNA was 1638 base pairs in length and contained 10 base pairs of poly (G) at the 5'-end followed by 71 base pairs of noncoding nucleotides. The next 78 base pairs coded for a leader sequence that was 26 amino acids in length and included a methionine start signal and a typical hydrophobic core. The following 1233 base pairs coded for 411 amino acids that are present in the mature protein followed by a stop codon of TAA, 207 base pairs of noncoding nucleotides, a poly (A) track of 15 base pairs, and 22base pairs of poly (C). Specific regions of the cDNA of the 7 chain were then compared with the cDNAs for the a and 8 chains of human fibrinogen. e 7 chain of human fibrinogen (Mr 46 500) consists of 411 amino acids (Lottspeich & Henschen, 1977; Henschen & Lottspeich, 1977) and a single carbohydrate chain linked to Asn-52 (Iwanaga et al., 1968). Two 7 chains, together with two a and two 8 chains, are cross-linked by intrachain disulfide bonds and form a distended polydomainal structure containing three nodules. An internal portion of the 7 chain (amino acid residues 24-134) is bounded by unique cystine residues and is located in the interdomainal connecting structure linking the three nodules (Doolittle et al., 1978). The carboxyl-ter-