Exonuclease 1-dependent and independent mismatch repair.
Exonuclease 1-dependent and independent mismatch repair.
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DOI：
10.1016/j.dnarep.2015.04.010
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发表时间：
2015-08
期刊：
影响因子：
3.8
通讯作者：
Kolodner RD
中科院分区：
文献类型：
--
作者：
Goellner EM;Putnam CD;Kolodner RD
DNA错配修复(MMR)作用于修复DNA复制过程中因错配错误而导致的错配碱基，并识别重组(HR)中间体中的错配碱基。外切酶1(Exo1)是一种5‘-3’外切酶，参与多种DNA修复途径。Exo1被鉴定为参与酿酒酵母和人类MMR的核酸外切酶，它的功能是在错配识别后切除子链，此外，Exo1还参与HR过程中的末端切除。然而，在活体HR过程中，Exo1并不是末端切除所绝对需要的。同样，虽然在体外重组的MMR反应中需要Exo1，但遗传学研究表明，在体内MMR并不是绝对需要的，这表明存在Exo1依赖和Exo1依赖的MMR亚通路。在这里，我们回顾了关于Exo1依赖和Exo1依赖亚途径的已知情况，包括对MMR基因突变的研究，这些突变特异性地破坏了这两个亚途径中的任何一个。
DNA mismatch repair (MMR) acts to repair mispaired bases resulting from misincorporation errors during DNA replication and also recognizes mispaired bases in recombination (HR) intermediates. Exonuclease 1 (Exo1) is a 5′ –3′ exonuclease that participates in a number of DNA repair pathways. Exo1 was identified as an exonuclease that participates in Saccharomyces cerevisiae and human MMR where it functions to excise the daughter strand after mispair recognition, and additionally Exo1 functions in end resection during HR. However, Exo1 is not absolutely required for end resection during HR in vivo. Similarly, while Exo1 is required in MMR reactions that have been reconstituted in vitro, genetics studies have shown that it is not absolutely required for MMR in vivo suggesting the existence of Exo1-independent and Exo1-dependent MMR subpathways. Here, we review what is known about the Exo1-independent and Exo1-dependent subpathways, including studies of mutations in MMR genes that specifically disrupt either subpathway.