Genetic structure and forensic parameters of 30 InDels for human identification purposes in 10 Tibetan populations of China
Genetic structure and forensic parameters of 30 InDels for human identification purposes in 10 Tibetan populations of China
复制标题
中国 10 个藏族群体中用于人类识别目的的 30 个 InDels 的遗传结构和法医参数。
DOI：
10.1016/j.fsigen.2019.02.002
复制
发表时间：
2019-05-01
影响因子：
3.1
通讯作者：
Hou, Yiping
中科院分区：
文献类型：
--
作者：
Li, Luyao;Ye, Yi;Hou, Yiping
插入/缺失分析在法医学研究中可以作为一种很有前途的有用的支持工具。Qigen Investigator(R)DIPplex Kit包含30个精心挑选的常染色体INDELs，目的是揭示群体遗传变异。本研究采用Investigator(R)DIPplex试剂盒对居住在中国不同地区的10个藏族人群进行了基因分型。测定等位基因频率和法医参数。经Bonferroni校正后，所有基因座/群体均未显著偏离硬Weinberg平衡(HWE)。10个藏族群体的综合匹配概率值在1.7148×10(-11)到5.3516×10(-1)度之间。我们的结果显示，中国的10个藏族群体在遗传上非常相似。利用系统聚类、多维尺度(MDS)、主成分分析(PCA)和结构分析等方法进行洲际种群分化分析表明，藏族种群与东亚种群具有较近的亲缘关系。全面的群体遗传学研究表明，30-INDELS分析在法医个人识别方面同样有效，可被视为亲属关系鉴定的有效补充方案。
Insertion/deletion analysis can serve as a promising and useful supporting tool in forensic research. The Qiagen Investigator (R) DIPplex Kit contained 30 well-chosen autosomal InDels was targeted to reveal the population genetic variation. In the present study, 10 Tibetan populations residing in different geographic areas of China were recruited and genotyped by Investigator (R) DIPplex Kit. Allele frequencies and forensic parameters were determined. No significant departures from Hard-Weinberg equilibrium (HWE) in all loci/populations after Bonferroni correction. The combined matching probability values range from 1.7148 x 10(-11) to 5.3516 x 10(-1)degrees in 10 Tibetan populations. Our results revealed 10 Tibetan populations in China are genetically very similar. Intercontinental population differentiation analysis indicated Tibetan populations had a close genetic relationship with East Asian populations using Hierarchical clustering, multi-dimensional scaling (MDS), principal component analysis (PCA) and STRUCTURE. Comprehensive population genetic studies revealed that the 30-InDels assay was similarly efficient in forensic personal identification and could be regarded as an effective supplementary protocol for kinship testing.