Development and characterization of three-dimensional layered structures with gel beads for bone tissue engineering
Development and characterization of three-dimensional layered structures with gel beads for bone tissue engineering
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DOI：
10.1016/j.rinma.2022.100317
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发表时间：
2022-12
影响因子：
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通讯作者：
Kurumi Takimoto;T. Arahira;M. Todo
中科院分区：
文献类型：
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作者：
Kurumi Takimoto;T. Arahira;M. Todo
我们设计了这项研究，以评估海藻酸凝胶珠，这是已知的，以促进增加保水性的应用。这些支架用相同材料的水溶液涂覆，在凝胶珠和海藻酸钠水溶液之间产生浓度梯度，其填充周围的3D层状结构以提高稳定性。本研究的目的是提高这些支架的机械性能和稳定性，以促进有效的增殖和分化在native setting.The三维凝胶珠反应海藻酸钠水溶液与氯化钙水溶液形成。通过直径和球形度评价凝胶珠。随着海藻酸钠浓度的增加，凝胶珠的直径增加，球形度变为约1。然后通过在24孔板上分层凝胶珠形成三维结构。利用万能试验机对凝胶珠三维层状结构进行了测试，并评价了其压缩力学性能。当凝胶珠的浓度和周围环境相同时，具有凝胶珠的三维层状结构的弹性模量最高。体外实验采用酶标仪测定细胞数和碱性磷酸酶活性。体外实验的结果表明，细胞数量与对照没有差异，但ALP活性倾向于较高的样品具有高弹性模量。结论：凝胶珠三维层状结构作为骨组织再生支架是有效的。
We designed this study to evaluate the application of alginic acid gel beads, which are known to facilitate increased water retention. These scaffolds were coated with an aqueous solution of the same material creating a concentration gradient between the gel beads and the sodium alginate aqueous solution, which fills the surrounding 3D layered structure to improve stability. The aim of this study was to enhance the mechanical properties and stability of these scaffolds to promote effective proliferation and differentiation in the native setting.The three-dimensional gel beads were formed by reacting a sodium alginate aqueous solution with a calcium chloride aqueous solution. The gel beads were evaluated by diameter and sphericity. As the concentration of sodium alginate increased, the diameter of the gel beads increased and sphericity became approximately 1. Then the three-dimensional structure was formed by layering the gel beads on a 24-well plate. The three-dimensional layered structures with gel beads were tested using a universal testing machine and evaluated compressive mechanical property. The modulus of elasticity for the three-dimensional layered structures with gel beads was the highest when the concentrations of the gel beads and surroundings were the same. In vitro experiment, the cell number and ALP activity were also measured by plate reader. The results of the in vitro experiments showed no difference in the cell numbers with the control, but the ALP activity tended to be higher with the samples having a high modulus of elasticity. It is concluded that three-dimensional layered structures with gel beads were effective for bone tissue regeneration as a scaffold.