Micromechanical valve-operated needle-on-a-chip microinjection module for microfluidic large-scale integration
Micromechanical valve-operated needle-on-a-chip microinjection module for microfluidic large-scale integration
复制标题
用于微流控大规模集成的微机械阀控针片微注射模块
DOI：
10.1088/1361-6439/ac984a
复制
发表时间：
2022
影响因子：
2.3
通讯作者：
Araci, I Emre
中科院分区：
文献类型：
--
作者：
Gray-Scherr, Delaney;Gasvoda, Hudson;Hadsell, Alex;Miller, Leilani;Demir, Ebru;Araci, I Emre
显微注射是基因工程中的一个基本过程，用于将遗传物质递送到各种生物样品中。考虑到从基因编辑到细胞治疗的显微注射应用的高通量要求，需要一种自动化、高度并行化、可重复且易于使用的显微注射策略。在这里，我们报告了一个芯片上，微流控微注射模块设计与微流控大规模集成技术，可以通过标准的，多层软光刻技术制造的兼容性。针芯片（NOC）模块由一个两层的聚二甲基硅氧烷为基础的微流体模块，其穿刺和注射操作是完全依赖于地震阀驱动。作为一个概念验证，我们设计了一个NOC模块来进行一个共同的遗传学模式生物，秀丽隐杆线虫（C。elegans）。使用有限元方法模拟分析了NOC设计的大范围的实际可行的几何参数。计算结果表明，控制沟道的微小横向偏移（> 10 μm）就足以在较大的制造公差（50 μm，50%沟道宽度）下成功实现NOC操作。为了证明概念验证，制造了显微注射平台并用于将示踪染料成功注射到C.优雅
Microinjection is an essential process in genetic engineering that is used to deliver genetic materials into various biological specimens. Considering the high-throughput requirement for microinjection applications ranging from gene editing to cell therapies, there is a need for an automated, highly parallelized, reproducible, and easy-to-use microinjection strategy. Here we report an on-chip, microfluidic microinjection module designed for compatibility with microfluidic large-scale integration technology that can be fabricated via standard, multilayer soft lithography techniques. The needle-on-chip (NOC) module consists of a two-layer polydimethylsiloxane-based microfluidic module whose puncture and injection operations are reliant solely on Quake valve actuation. As a proof-of-concept, we designed a NOC module to conduct the microinjection of a common genetics model organism, Caenorhabditis elegans (C. elegans). The NOC design was analyzed using finite element method simulations for a large range of practically viable geometrical parameters. The computational results suggested that a slight lateral offset (> 10 μm) of the control channel is sufficient for a successful NOC operation with a large fabrication tolerance (50 μm, 50% channel width). To demonstrate proof-of-concept, the microinjection platform was fabricated and utilized to perform a successful injection of a tracer dye into C. elegans.