Estimation of the effective orientation of the SHG source in primary cortical neurons
Estimation of the effective orientation of the SHG source in primary cortical neurons
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DOI：
10.1364/oe.17.014418
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发表时间：
2009-08-03
期刊：
影响因子：
3.8
通讯作者：
Loza-Alvarez, Pablo
中科院分区：
文献类型：
--
作者：
Psilodimitrakopoulos, Sotiris;Petegnief, Valerie;Loza-Alvarez, Pablo
在本文中，我们提供了，第一次我们的知识，在体外培养的皮层神经元过程中的SHG源的有效方向。这是通过使用偏振敏感的二次谐波产生（PSHG）成像显微镜技术。通过执行像素级分辨率分析，我们发现SHG偶极子源具有以θ（e）= 33.96度为中心的角度分布，带宽为Delta θ（e）= 12.85度。这种取向可能与微管中微管蛋白异二聚体的分子几何结构有关。(C)2009年美国光学学会
In this paper we provide, for the first time to our knowledge, the effective orientation of the SHG source in cultured cortical neuronal processes in vitro. This is done by the use of the polarization sensitive second harmonic generation (PSHG) imaging microscopy technique. By performing a pixel-level resolution analysis we found that the SHG dipole source has a distribution of angles centered at theta(e) = 33.96 degrees, with a bandwidth of Delta theta(e) = 12.85 degrees. This orientation can be related with the molecular geometry of the tubulin heterodimmer contained in microtubules. (C) 2009 Optical Society of America