Synthetic peptide-acrylate surfaces for long-term self-renewal and cardiomyocyte differentiation of human embryonic stem cells
Synthetic peptide-acrylate surfaces for long-term self-renewal and cardiomyocyte differentiation of human embryonic stem cells
复制标题
DOI：
10.1038/nbt.1629
复制
发表时间：
2010-06-01
影响因子：
46.9
通讯作者：
Brandenberger, Ralph
中科院分区：
文献类型：
--
作者：
Melkoumian, Zara;Weber, Jennifer L.;Brandenberger, Ralph
人类胚胎干细胞（hESCs）具有两种特性：自我更新和分化为人体所有主要体细胞谱系的潜力。hesc衍生细胞的广泛临床应用将需要低成本、稳健、可扩展和使用化学原料的培养方法。在这里，我们描述了合成的肽-丙烯酸酯表面（PAS），支持hESCs在化学定义的，无xeno介质中的自我更新。H1和H7 hESCs在PAS上成功维持了十余代。在PAS和Matrigel上培养的细胞形态和表型标记表达相似。在PAS上的细胞保留了正常的核型和多能性，并能够分化为功能心肌细胞。最后，将PAS扩展到大型培养容器格式。支持hESCs自我更新和分化的合成、无xeno、可扩展表面将有助于研究目的和细胞治疗的发展。
Human embryonic stem cells (hESCs) have two properties of interest for the development of cell therapies: self-renewal and the potential to differentiate into all major lineages of somatic cells in the human body. Widespread clinical application of hESC-derived cells will require culture methods that are low-cost, robust, scalable and use chemically defined raw materials. Here we describe synthetic peptide-acrylate surfaces (PAS) that support self-renewal of hESCs in chemically defined, xeno-free medium. H1 and H7 hESCs were successfully maintained on PAS for over ten passages. Cell morphology and phenotypic marker expression were similar for cells cultured on PAS or Matrigel. Cells on PAS retained normal karyotype and pluripotency and were able to differentiate to functional cardiomyocytes on PAS. Finally, PAS were scaled up to large culture-vessel formats. Synthetic, xeno-free, scalable surfaces that support the self-renewal and differentiation of hESCs will be useful for both research purposes and development of cell therapies.