DISTRIBUTION, MORPHOLOGY AND NUMBER OF MONOAMINE-SYNTHESIZING AND SUBSTANCE P-CONTAINING NEURONS IN THE HUMAN DORSAL RAPHE NUCLEUS
DISTRIBUTION, MORPHOLOGY AND NUMBER OF MONOAMINE-SYNTHESIZING AND SUBSTANCE P-CONTAINING NEURONS IN THE HUMAN DORSAL RAPHE NUCLEUS
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DOI：
10.1016/0306-4522(91)90043-n
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发表时间：
1991-01-01
期刊：
影响因子：
3.3
通讯作者：
TORK, I
中科院分区：
文献类型：
--
作者：
BAKER, KG;HALLIDAY, GM;TORK, I
本文研究了人中缝背核内含5-羟色胺、儿茶酚胺和P物质神经元的分布、形态和数量。平行系列切片取自10例尸检的人类脑组织，这些脑组织来自年龄在42岁到88岁之间的非神经系统疾病患者。用抗苯丙氨酸羟基酶(含色氨酸羟基酶的5-羟色胺神经元)、酪氨酸羟基酶(儿茶酚胺神经元)和P物质的免疫组织化学方法对神经元进行免疫组织化学鉴定，并制备了Nissl染色的参考切片，并利用单独发表的数据来描绘中缝背核的亚核分裂，以确定每个亚核中的神经元总数。(1)中缝背核各区均有5-羟色胺合成神经元，总数为165,000+/-34,000个。五个亚核(尾侧亚核、背侧亚核、腹侧亚核、腹外侧亚核和束间亚核)的神经元类型与Nissl材料中的神经元类型相同。(2)含P物质的神经元主要分布在核嘴部，数目为74,600+/-17,600个。(3)儿茶酚胺细胞仅见于中缝背核吻部，其数目为5600+/-3400个。(4)在中缝背核腹侧亚核和束间亚核中，5-羟色胺合成神经元和P物质神经元的数目之和超过了NSL染色神经元的总数，表明中缝背核有相当一部分细胞群同时存在5-羟色胺和P物质。这些神经元的形态和大小高度相似，这进一步支持了这一点。结论是，人类中缝背核的不同亚区之间存在明显的化学差异。这些差异与动物行为学研究的结果是一致的，这些研究表明，从这个核团中出现了不同的投射路径。
The distribution, morphology and number of serotonin-, catecholamine- and substance P-containing neurons in the human dorsal raphe nucleus were studied. Parallel series of sections were prepared from 10 human brainstems obtained at autopsy from patients without neurological disease aged between 42 and 88 years. The neurons were identified using immunohistochemistry with antibodies raised against phenylalanine hydroxylase (tryptophan hydroxylase-containing, serotonin neurons), tyrosine hydroxylase (catecholamine neurons) and substance P. A reference series of Nissl-stained sections was also prepared and data published separately were used to delineate the subnuclear divisions of the dorsal raphe nucleus and to establish the total number of neurons in each subnucleus.The following principal findings emerged. (1) Serotonin-synthesizing neurons are present in all regions of the dorsal raphe nucleus and their total number is 165,000 +/- 34,000. The same types of neurons as those seen in Nissl material characterize each of the five subnuclei (caudal, dorsal, ventral, ventrolateral and interfascicular). (2) Substance P-containing neurons mostly occupy the rostral part of the nucleus and their number is 74,600 +/- 17,600. (3) Catecholamine cells are only found in the rostral part of the dorsal raphe nucleus and their number is 5600 +/- 3400. (4) In the ventral and interfasicular subnuclei the combined number of serotonin-synthesizing and substance P-containing neurons exceeds the total number of Nssl-stained neurons suggesting that serotonin and substance P co-exist in a substantial part of the cell population of the dorsal raphe nucleus. This is further supported by the highly similar morphology and size of these neurons.It is concluded that there are demonstrable chemical differences between the various subregions of the human dorsal raphe nucleus. These differences are in harmony with the results of hodological studies in animals, which have demonstrated differential projection pathways emerging from this nucleus.