Differential expression of growth differentiation factor-9 in keloids
Differential expression of growth differentiation factor-9 in keloids
复制标题
生长分化因子9在瘢痕疙瘩中的差异表达
DOI：
10.1016/j.burns.2010.02.009
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发表时间：
2010-12-01
期刊：
影响因子：
2.7
通讯作者：
Liu, Wei
中科院分区：
文献类型：
--
作者：
Gao, Zhen;Wu, Xiaoli;Liu, Wei
目的瘢痕疙瘩的侵袭机制尚不清楚，本研究旨在分析瘢痕疙瘩周边区和中心区的差异表达基因，从而确定可能与瘢痕疙瘩侵袭有关的分子。（TGF β）应用超家族信号通路分析3例外周区和中央区成纤维细胞的差异表达基因结果瘢痕疙瘩成纤维细胞中GDF 9的表达水平在瘢痕疙瘩的周边部明显高于中央部，而在增生性瘢痕成纤维细胞中GDF 9的表达水平在增生性瘢痕的周边部明显高于中央部瘢痕疙瘩组织中GDF 9的表达水平明显高于增生性瘢痕和正常皮肤组织（p < 0 0 5）。结论GDF 9在瘢痕疙瘩周围组织中的表达上调可能与瘢痕疙瘩的侵袭行为有关。
Objective The mechanisms of keloid invasion are largely unknown This study aims to analyze the differentially expressed genes between keloid peripheral and central areas and thus to define the molecule that might be responsible for keloid invasionMethods The gene chip of transforming growth factor beta (TGF beta) superfamily signaling pathway was used to analyze differentially expressed genes of the fibroblasts derived from peripheral area and central area of 3 keloids The differential expression of growth differ entiation factor 9 (GDF 9) was also confirmed by quantitative PCR and Western blot Moreover GDF 9 expression levels were compared among the fibroblasts derived from 3 keloids hypertrophic scars and normal skin with quantitative PCR and immunofluorescent stainingResults GDF 9 expression level was significantly higher in the peripheral area than in the central area (p < 0 05) and was significantly higher in keloid than in hypertrophic scar and normal skin (p < 0 05)Conclusion Up regulated GDF 9 expression in keloid peripheral area may play a role in keloid invasive behavior (C) 2010 Elsevier Ltd and ISBI All rights reserved