REGULATION OF ALCOHOL-DEHYDROGENASE IN DROSOPHILA-MELANOGASTER BY DIETARY ALCOHOL AND CARBOHYDRATE
REGULATION OF ALCOHOL-DEHYDROGENASE IN DROSOPHILA-MELANOGASTER BY DIETARY ALCOHOL AND CARBOHYDRATE
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DOI：
10.1016/0020-1790(84)90034-9
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发表时间：
1984-01-01
期刊：
影响因子：
--
通讯作者：
GEER, BW
中科院分区：
文献类型：
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作者：
MCKECHNIE, SW;GEER, BW
喂给D.在含有0.5%（wt/vol）蔗糖、2.5%（vol/vol）乙醇的Sang ′ s改良培养基C上无菌培养的黑腹Canton-S野生型幼虫诱导的醇脱氢酶（ADH）活性比饲喂未补充的0.5%蔗糖饮食的幼虫中的活性高至少2.5倍。1.1.1.1乙醇仅诱导ADH交叉反应物质（CRM）的2倍变化，表明ADH也存在翻译后修饰。添加膳食蔗糖的饮食（在5%，重量/体积）产生类似的，但较小，ADH活性和CRM的影响。在2.5%乙醇存在下，增加膳食蔗糖至5%，降低ADH活性和CRM至低于0.5%蔗糖-2.5%乙醇饮食中达到的水平。经检查，饮食乙醇降低了ADH-1的相对浓度，增加了ADH-3和ADH-5的浓度在幼虫中，蔗糖有类似的，但较小的影响，同工酶模式。由于饲料乙醇增加了总NAD含量和NADH/NAD+比值，因此建议将饲料诱导的同工酶谱变化与幼虫组织中NADH、NAD+和ADH酶的相对量联系起来。幼虫ADH活性受饮食酒精和碳水化合物的调节。
When fed to D. melanogaster Canton-S wild-type larvae cultured axenically on Sang''s modified medium C with 0.5% (wt/vol) sucrose, 2.5% (vol/vol) ethanol induced an alcohol dehydrogenase (ADH) [EC 1.1.1.1] activity at least 2.5-fold higher than the activity in larvae fed an unsupplemented 0.5% sucrose diet. Ethanol induced only a 2-fold change in ADH cross reacting material (CRM), suggesting that there was also a post-translational modification of ADH. The addition of dietary sucrose to the diet (at 5%, wt/vol) exerted similar, but lesser, effects on ADH activity and CRM. Increasing dietary surcose to 5% in the presence of 2.5% ethanol decreased ADH activity and CRM to levels below those attained in the 0.5% sucrose-2.5% ethanol diet. Upon examination, dietary ethanol decreased the relative concentration of ADH-1 and increased the concentrations of ADH-3 and ADH-5 in larvae; sucrose had a similar, but lesser, effect on the isozyme pattern. Because dietary ethanol increased both the total NAD content and the NADH/NAD+ ratio, the diet-induced changes in isozyme pattern were proposed to be linked to the relative amounts of NADH, NAD+ and ADH enzyme in larval tissues. Larval ADH activity is modulated by both dietary alcohol and carbohydrate.