Cross-validation of the RT-QuIC assay for the antemortem detection of chronic wasting disease in elk
Cross-validation of the RT-QuIC assay for the antemortem detection of chronic wasting disease in elk
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DOI：
10.1080/19336896.2020.1716657
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发表时间：
2020-01-01
期刊：
影响因子：
2.3
通讯作者：
Thomsen, B., V
中科院分区：
文献类型：
--
作者：
Haley, N. J.;Donner, R.;Thomsen, B., V
慢性消耗性疾病是一种渐进性致命的，水平传播的朊病毒疾病，影响几个成员的鹿科物种。常规诊断依赖于使用死后收集的组织进行ELISA或IHC评估;然而，最近的研究集中在使用死前收集的样本的新开发的扩增技术上。本研究旨在交叉验证从患有地方性慢性消耗病的麋鹿群收集的直肠活检的实时震动诱导转换试验（RT-QuIC）评价，评估二元阳性/阴性检测结果以及实验室之间的相对扩增率。我们发现，在所有进行RT-QuIC的实验室中，结果在两个类别中都是相关的，并且与在国家参考实验室进行的常规IHC相关。使用RT-QuIC鉴定出的阳性样本数量显著增加，结果似乎不受低卵泡计数的影响。这些发现支持继续开发和实施扩增检测方法，用于诊断兽医重要的朊病毒疾病，不仅针对死前采样策略，还针对死后检测方法。
Chronic wasting disease is a progressively fatal, horizontally transmissible prion disease affecting several members of the cervid species. Conventional diagnosis relies on ELISA or IHC evaluation using tissues collected post-mortem; however, recent research has focused on newly developed amplification techniques using samples collected antemortem. The present study sought to cross-validate the real-time quaking-induced conversion assay (RT-QuIC) evaluation of rectal biopsies collected from an elk herd with endemic CWD, assessing both binary positive/negative test results as well as relative rates of amplification between laboratories. We found that results were correlative in both categories across all laboratories performing RT-QuIC, as well as to conventional IHC performed at a national reference laboratory. A significantly higher number of positive samples were identified using RT-QuIC, with results seemingly unhindered by low follicle counts. These findings support the continued development and implementation of amplification assays in the diagnosis of prion diseases of veterinary importance, targeting not just antemortem sampling strategies, but post-mortem testing approaches as well.