Numbers and Biomass of Bacteria and Fungi Obtained by the Direct Microscopic Count Method
Numbers and Biomass of Bacteria and Fungi Obtained by the Direct Microscopic Count Method
复制标题
直接显微镜计数法获得的细菌和真菌的数量和生物量
DOI：
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发表时间：
2012
期刊：
影响因子：
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通讯作者：
S. Aburatani
中科院分区：
文献类型：
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作者：
A. Itsuki;S. Aburatani
The soil ecology of the organic and mineral soil layers of laurel-leaved and Cryptomeria japonica forest in the Kasuga-yama Hill Primeval Forest (Nara, Japan) was assessed. The number of bacteria obtained by the dilution plate count method was less than 0.05% of those counted by the direct microscopic count. We therefore found that forest soil contains large numbers of non-culturable bacteria compared with agricultural soils. The numbers of bacteria and fungi obtained by both the dilution plate count and the direct microscopic count were larger in the deeper horizons (F and H) of the organic layer than in the mineral soil layer. This suggests that active microbial metabolism takes place in the organic layer. The numbers of bacteria and the length of fungal hyphae obtained by the direct count method were greater in the H horizon than in the F horizon. The direct microscopic count revealed numerous non-culturable bacteria and fungi in the soil. The ratio of fungal to bacterial biomass was lower in the laurel-leaved forest soil. The fungal biomass was therefore relatively low in the laurel-leaved forest soil due to differences in forest vegetation. Keywords—Bacterial number, Dilution plate count, Direct microscopic count, Forest soil.
