Mesenchymal Stem Cells Sense the Toughness of Nanomaterials and Interfaces
Mesenchymal Stem Cells Sense the Toughness of Nanomaterials and Interfaces
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DOI：
10.1002/adhm.202203297
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发表时间：
2022-04
期刊：
影响因子：
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通讯作者：
Li-ping Peng;Carlos Matellan;Armando E. del Río Hernández;J. Gautrot
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文献类型：
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作者：
Li-ping Peng;Carlos Matellan;Armando E. del Río Hernández;J. Gautrot
已知干细胞能够感知并响应来自其细胞外环境的广泛的物理刺激。特别是，生物材料的机械性能（杨氏或剪切模量，粘弹性）的作用已被广泛证明对干细胞的粘附，扩散，扩增和分化有显着的影响。反过来，细胞对其环境施加力，这可能导致相关组织的形状、大小和收缩发生显著变化，并可能导致机械破坏和功能故障。然而，到目前为止，还没有研究将干细胞表型与生物材料韧性相关联。实际上，将韧性介导的细胞响应与其他机械感测过程分离仍然是难以捉摸的，因为在与细胞产生的力相关的范围内将杨氏模量或剪切模量与韧性分离是特别具有挑战性的。在这份报告中，我们展示了聚合物纳米片的大分子结构的设计如何调节界面韧性，独立于界面剪切储能模量，以及这反过来又如何控制间充质干细胞在液体界面的扩张。
Stem cells are known to sense and respond to a broad range of physical stimuli arising from their extra-cellular environment. In particular, the role of the mechanical properties (Youngs or shear modulus, viscoelasticity) of biomaterials has extensively been shown to have a significant impact on the adhesion, spreading, expansion and differentiation of stem cells. In turn, cells exert forces on their environment that can lead to striking changes in shape, size and contraction of associated tissues, and may result in mechanical disruption and functional failure. However, no study has so far correlated stem cell phenotype and biomaterials toughness. Indeed, disentangling toughness-mediated cell response from other mechanosensing processes has remained elusive as it is particularly challenging to uncouple Youngs’ or shear moduli from toughness, within a range relevant to cell-generated forces. In this report, we show how the design of macromolecular architecture of polymer nanosheets regulates interfacial toughness, independently to interfacial shear storage modulus, and how this, in turn, controls the expansion of mesenchymal stem cells at liquid interfaces.