Comparison of Plasma Membrane Proteomic Changes of Arabidopsis Suspension-Cultured Cells (T87 Line) after Cold and ABA Treatment in Association with Freezing Tolerance Development
Comparison of Plasma Membrane Proteomic Changes of Arabidopsis Suspension-Cultured Cells (T87 Line) after Cold and ABA Treatment in Association with Freezing Tolerance Development
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DOI：
10.1093/pcp/pcs010
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发表时间：
2012-03-01
影响因子：
4.9
通讯作者：
Uemura, Matsuo
中科院分区：
文献类型：
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作者：
Li, Bin;Takahashi, Daisuke;Uemura, Matsuo
质膜是植物遭受冻害的主要部位。为了确定与抗冻性发展相关的PM蛋白质谱的总体变化，采用无标记蛋白质定量技术对拟南芥悬浮培养细胞（T87系）的纯化PM进行了蛋白质组分析。低温驯化（CA）或阿坝处理后，生长停滞期（8 d龄）的拟南芥细胞的抗冻性增强。蛋白质组分析共分配了658个蛋白质，其中45.3%（298个蛋白质）预测具有跨膜结构域。它们被分为几个功能类别，主要类别是转运蛋白，信号转导，蛋白质目的地和储存以及细胞结构。CA后，271个蛋白质增加，111个蛋白质减少。阿坝处理导致185种蛋白质含量增加，56种蛋白质含量减少。其中，139个增加和49个减少的蛋白质在CA和阿坝处理后被鉴定为共同的。此外，在冷处理（132个增加和62个减少）或阿坝处理（46个增加和7个减少）的细胞中有特异性表达的蛋白质。总的来说，我们的研究结果清楚地表明，（i）PM蛋白质组对CA和阿坝处理的反应基本上重叠，但同时，一些蛋白质在每种处理中表现出不同的反应模式;（ii）大多数ABA反应蛋白是CA反应蛋白，但不是相反，这表明CA和阿坝信号通路在PM蛋白质组反应中的复杂相互作用。
The plasma membrane (PM) is the primary site of freezing injury in plants. To determine global changes in PM protein profiles in association with freezing tolerance development, proteome analysis of the purified PM of Arabidopsis suspension-cultured cells (T87 line) was conducted with label-free protein quantification technology. Freezing tolerance of Arabidopsis cells at the lag growth phase (8 d old) increased after cold acclimation (CA) or ABA treatment. Proteome analysis assigned 658 proteins in the PM in total, of which 45.3% (298 proteins) were predicted to have transmembrane domains. They were classified into several functional categories, with the primary categories being proteins in transporters, signal transduction, protein destination and storage, and cell structure. After CA, 271 proteins increased and 111 proteins decreased. ABA treatment resulted in 185 increased and 56 decreased proteins. Of these, 139 increased and 49 decreased proteins were identified in common after both CA and ABA treatment. In addition, there were proteins specifically expressed in cold- (132 increased and 62 decreased) or ABA- (46 increased and 7 decreased) treated cells. Collectively, our results clearly show that (i) responses of the PM proteome to CA and ABA treatment overlap substantially but, at the same time, some proteins exhibited different response patterns in each treatment; and (ii) the majority of ABA-responsive proteins are CA-responsive proteins but not vice versa, suggesting complex interactions of CA and ABA signaling pathways in the PM proteome responses.