Methylation of thymine residues during oligonucleotide synthesis.
Methylation of thymine residues during oligonucleotide synthesis.
复制标题
寡核苷酸合成过程中胸腺嘧啶残基的甲基化。
DOI：
10.1093/nar/13.2.573
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发表时间：
1985
影响因子：
14.9
通讯作者：
Jones,RA
中科院分区：
文献类型：
--
作者：
Gao,X;Gaffney,BL;Senior,M;Riddle,RR;Jones,RA
寡脱氧核苷酸中的胸腺嘧啶残基通过核苷酸间的甲基磷酸三酯键在N3处发生甲基化。这种烷基化在强碱如DBU存在下发生得最快，但在寡核苷酸合成期间也以慢得多的速率发生。
Thyinine residues in an oligodeoxyribonucleotide are subject to methylation at N3by the internucleotide methyl phosphotriester linkages. This alkylation occurs most rapidly in the presence of a strong base such as DBU, but also takes place, at a much slower rate, during oligonucleotide synthesis.