Soluble plasma antigen in experimental Salmonella typhimurium infection in mice.
Soluble plasma antigen in experimental Salmonella typhimurium infection in mice.
复制标题
小鼠实验性鼠伤寒沙门氏菌感染中的可溶性血浆抗原。
DOI：
10.1111/j.1574-695x.1994.tb00368.x
复制
发表时间：
1994
影响因子：
--
通讯作者：
Hentges,DJ
中科院分区：
文献类型：
--
作者：
Butler,T;Rolfe,RD;James,GM;Hentges,DJ
为检测和鉴定血液中沙门氏菌抗原，将远交CF-1雌性小鼠腹腔接种沙门氏菌。用ELISA法检测鼠伤寒沙门氏菌LT-2和血中沙门氏菌共同结构抗原。血浆抗原在感染过程的早期可检测到，并且在疾病过程的后期，当动物表现出高度菌血症和脾细菌计数高时，血浆抗原的数量增加。将抗原与血液的无细胞血浆部分结合，通过截留分子量为0.2 μ和1000 kDa的过滤器，并在加热至100 ° C持续15 min后增强可检测性。通过在0.1 M EDTA中以1：4稀释血浆，加热至100 ° C，并用截留分子量为15 kDa的超滤膜浓缩上清液来浓缩抗原。通过凝胶过滤，在加热的血浆中抗原与约300 kDa分子量处的峰相关，在未加热的血浆中与约380 kDa的峰相关。这些结果表明，鼠伤寒感染导致循环可溶性血浆抗原，其是热稳定的，分子量约为300 kDa。
To detect and characterizeSalmonellaantigen in blood, outbred CF-1 female mice were inoculated intraperitoneally withS. typhimuriumLT-2 and blood was assayed by ELISA forSalmonellacommon structural antigen. Plasma antigen was detectable early in the course of infection and increased in quantity later in the course of illness when animals showed high grade bacteremia and high counts of splenic bacteria. Antigen was associated with a cell-free plasma fraction of blood, passed through filters with cut-offs of 0.2 μ and molecular mass of 1000 kDa, and was enhanced in detectability after heating to 100°C for 15 min. Antigen was concentrated by diluting plasma 1:4 in 0.1 M EDTA, heating to 100°C, and concentrating the supernate with an ultrafiltration membrane with a molecular mass cut-off of 15 kDa. By gel filtration, antigen was associated with a peak at about molecular mass 300 kDa in heated plasma and a peak at about 380 kDa in unheated plasma. These results indicate that murine typhoid infection results in circulating soluble plasma antigen, which is heat-stable with a molecular mass of approximately 300 kDa.