Sympathetic Regulation of Tertiary Dentinogenesis via Beta-2 Adrenergic Receptor on Rat Odontoblasts
Sympathetic Regulation of Tertiary Dentinogenesis via Beta-2 Adrenergic Receptor on Rat Odontoblasts
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DOI：
10.1016/j.joen.2015.01.010
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发表时间：
2015-07-01
影响因子：
4.2
通讯作者：
Suda, Hideaki
中科院分区：
文献类型：
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作者：
Gu, Jie;Ikeda, Hideharu;Suda, Hideaki
简介：在成骨细胞膜内发现了 Beta-2 肾上腺素能受体，介导骨重塑。普萘洛尔是一种交感神经β拮抗剂，通常用作治疗高血压等许多常见疾病的长期药物。这项研究的目的是验证大鼠成牙本质细胞上是否存在这种受体，如果存在，则研究其与第三期牙本质发生的可能关联。方法：20只雄性Sprague-Dawley大鼠（9周龄）随机分为4组：CP0.8组，空腔准备+普萘洛尔治疗（0.8 mg/天，n=5）； CP4组，腔体准备+普萘洛尔治疗（4.0mg/天，n=7）； CON组，腔准备+盐水治疗（0.2 mUday，n = 5）； NT组，不进行治疗（n = 3）。在双侧上颌第一磨牙近中面进行蛀牙预备。 2周后，通过苏木精-伊红染色检查第三牙本质发生（CP0.8、CP4和CON），并通过免疫组织化学检查β2肾上腺素受体（NT）的定位。结果：在正常大鼠磨牙牙髓的成牙本质细胞层中观察到β2肾上腺素受体免疫反应性。与未接受普萘洛尔治疗的大鼠相比，接受两周普萘洛尔全身给药的大鼠在准备好的空腔下的第三级牙本质发生显着更高。普萘洛尔的高剂量治疗（P < .001）比低剂量治疗（P < .01）更有效地上调第三级牙本质发生。结论：这些结果表明，交感神经系统通过位于大鼠成牙本质细胞上的 β2 肾上腺素能受体减少第三级牙本质的形成。提示β肾上腺素能拮抗剂有望用于诱导三级牙本质形成以保护牙髓的治疗。
Introduction: Beta-2 adrenergic receptor has been found within the osteoblast membrane meditating bone remodeling. Propranolol is a sympatholytic beta antagonist commonly used as long-term medication for the management of many common diseases such as hypertension. This study was performed to verify the presence of this receptor on odontoblasts in rats and, if present, to investigate its possible association with tertiary dentinogenesis. Methods: Twenty male Sprague-Dawley rats (9 weeks old) were randomly assigned to 4 groups: CP0.8 group, cavity preparation + propranolol treatment (0.8 mg/day, n = 5); CP4 group, cavity preparation + propranolol treatment (4.0 mg/day, n = 7); CON group, cavity preparation + saline treatment (0.2 mUday, n = 5); and NT group, no treatment (n = 3). Cavity preparation was performed on the mesial aspect of the maxillary first molars bilaterally. After 2 weeks, the tertiary dentinogenesis (CP0.8, CP4, and CON) was examined by hematoxylin-eosin staining, and the localization of beta-2 adrenergic receptor (NT) was examined by immunohistochemistry. Results: The beta-2 adrenergic receptor immunoreactivity was observed in the odontoblastic layer in normal rat molar dental pulp. The tertiary dentinogenesis beneath the prepared cavity was significantly higher in the rats receiving 2-week systemic administration of propranolol than in those without the propranolol treatment. The higher-dose treatment of propranolol (P < .001) presented more effective up-regulation of tertiary dentinogenesis than the lower-dose treatment (P < .01). Conclusions: These results indicate that the sympathetic nervous system decreases tertiary dentin formation via beta-2 adrenergic receptors located on rat odontoblasts. It suggests that adrenergic beta antagonist is expected to use in the treatment of inducing tertiary dentin formation to protect dental pulp.