Anti-oxidative, anti-inflammatory and hepatoprotective effects of Radix Bupleuri extract against oxidative damage in tilapia (Oreochromis niloticus) via Nrf2 and TLRs signaling pathway
Anti-oxidative, anti-inflammatory and hepatoprotective effects of Radix Bupleuri extract against oxidative damage in tilapia (Oreochromis niloticus) via Nrf2 and TLRs signaling pathway
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DOI：
10.1016/j.fsi.2019.07.080
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发表时间：
2019-10-01
影响因子：
4.7
通讯作者：
Yin, Guojun
中科院分区：
文献类型：
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作者：
Jia, Rui;Gu, Zhengyan;Yin, Guojun
柴胡提取物(RBE)是我国最受欢迎的中草药之一，具有抗氧化、抗炎等功效。然而，其对罗非鱼氧化损伤的保护作用及其潜在的分子机制仍不清楚。本研究的目的是探索RBE的抗氧化、抗炎和抗氧化损伤的肝保护作用，并阐明其在鱼类中的潜在分子机制。在罗非鱼饲料中添加0、1、3g/kg的RBE，连续60d后，分别给罗非鱼腹腔注射过氧化氢或生理盐水。注射前，RBE处理改善了罗非鱼的生长性能和部分抗氧化能力。氧化损伤后，RBE预处理能显著降低血清中较高的转氨酶、碱性磷酸酶(AKP)和肝坏死。在血清和肝脏中，RBE可恢复由H_2O_2引起的异常脂质过氧化水平和抗氧化状态。此外，RBE处理激活了红系2相关因子2(NRF2)信号通路，促进了HO-1、NAD(P)H：QO-1、谷胱甘肽-S转移酶(GST)和过氧化氢酶(CAT)的基因表达。同时，RBE治疗通过抑制TLRs-MyD88-NF-kappa B信号通路减轻炎症反应，同时降低IL-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)和IL-8mRNA水平。此外，RBE处理上调了补体(C3)基因的表达，下调了热休克蛋白(HSP70)的基因表达。综上所述，目前的研究表明，RBE预处理对过氧化氢诱导的罗非鱼氧化损伤具有保护作用。RBE的有益活性可能是通过调节Nrf2/ARE和TLRs-MyD88-NF-kappaB信号通路实现的。
Radix Bupleuri extract (RBE) is one of the most popular oriental herbal medicines, which has anti-oxidative and anti-inflammatory properties. However, its protective effects and underlying molecular mechanisms on oxidative damage in tilapia are still unclear. The aims of the study were to explore the anti-oxidative, anti-inflammatory and hepatoprotective effects of RBE against oxidative damage, and to elucidate underlying molecular mechanisms in fish. Tilapia received diet containing three doses of RBE (0, 1 and 3 g/kg diet) for 60 days, and then were given an intraperitoneal injection of H2O2 or saline. Before injection, RBE treatments improved growth performance and partial anti-oxidative capacity in tilapia. After oxidative damage, RBE pretreatments were able to signally reduce the higher serum aminotransferases, alkaline phosphatase (AKP) and liver necrosis. In serum and liver, the abnormal lipid peroxidation level and antioxidant status induced by H2O2 injection were restored by RBE treatments. Furthermore, RBE treatments activated erythroid 2-related factor 2 (Nrf2) signaling pathway, which promoted the gene expression of heme oxygenase 1 (HO-1), NAD(P) H:quinone oxidoreductase 1 (NQO-1), glutathione-S-transferase (GST) and catalase (CAT). Meanwhile, RBE treatments reduced inflammatory response by inhibiting TLRs-MyD88-NF-kappa B signaling pathway, accompanied by the lower interleukin-1 beta (IL-1 beta), tumor necrosis factor-alpha (TNF-alpha) and IL-8 mRNA levels. In addition, RBE treatments upregulated complement (C3) gene expression and downregulated heat shock protein (HSP70) gene expression. In conclusion, the current study suggested that RBE pretreatments protected against H2O2-induced oxidative damage in tilapia. The beneficial activity of RBE may be due to the modulation of Nrf2/ARE and TLRs-Myd88-NF-kappa B signaling pathway.