Effects of Lycium barbarum polysaccharides on oxidative stress in hyperlipidemic mice following chronic composite psychological stress intervention.
Effects of Lycium barbarum polysaccharides on oxidative stress in hyperlipidemic mice following chronic composite psychological stress intervention.
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DOI：
10.3892/mmr.2014.3128
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发表时间：
2015-05
影响因子：
3.4
通讯作者：
Xiaodan Zhu;Shuting Hu;Lingqin Zhu;Juan Ding;Yong-can Zhou;Guanghua Li
中科院分区：
文献类型：
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作者：
Xiaodan Zhu;Shuting Hu;Lingqin Zhu;Juan Ding;Yong-can Zhou;Guanghua Li
慢性复合型心理应激干预是指对可能导致心理应激的因素进行累积，包括食物剥夺、缺水、冷水游泳等。大约40%的动脉粥样硬化病例与慢性复合心理应激有关。本研究旨在探讨枸杞多糖(LBP)对慢性复合心理应激所致高脂血症小鼠血脂水平和氧化应激的影响。建立高脂血症小鼠模型，给予慢性复合心理应激，给予枸杞多糖治疗30d。30d后测定血清甘油三酯(TG)和总胆固醇(TC)水平，并检测肝脏胆固醇7α-羟基酶(CyP7A1mRNA)的表达水平，观察脂质代谢的变化。测定肝组织超氧化物歧化酶(SOD)和丙二醛(MDA)水平，评价LBP对氧化应激的影响。测定大鼠血清白介素6(IL-6)和热休克蛋白70(HSP-70)水平，以评价主动脉炎症反应的程度，以及LBP的保护作用。慢性复合心理应激(HS)组小鼠血清甘油三酯(TG)、总胆固醇(TC)、丙二醛(MDA)和白介素6(IL-6)水平显著高于单纯应激组(HL)和LBP加应激组(HLS)。此外，HS组与HL组和HLS组比较，其血清中的SOD、HSP-70水平及细胞色素P7A1基因的表达水平均显著降低。这些结果提示，慢性复合心理应激可能通过诱导主动脉炎症反应和脂质过氧化，促进动脉粥样硬化的发生和发展。此外，LBP治疗显著抑制氧化应激和主动脉炎症反应。
Chronic composite psychological stress intervention is the accumulation of factors which may induce psychological stress, including food deprivation, water deprivation and swimming in cold water. Approximately 40% of cases of atherosclerosis are associated with chronic composite psychological stress. The aim of the present study was to explore the effects of Lycium barbarum polysaccharides (LBP) on blood lipid levels and oxidative stress in hyperlipidemic mice, following chronic composite psychological stress. A hyperlipidemic mouse model was generated, and the mice were subjected to chronic composite psychological stress and treated with LBP for 30 days. After 30 days the triglyceride (TG) and total cholesterol (TC) levels were measured in the serum, and the mRNA expression levels of cholesterol 7α‑hydroxylase (CYP7A1) were determined in the liver, in order to observe any changes to lipid metabolism. The levels of superoxide dismutase (SOD) and malondialdehyde (MDA) were measured in the liver to evaluate the effects of LBP on oxidative stress. The blood serum levels of interleukin‑6 (IL‑6) and heat shock protein 70 (HSP‑70) were measured to evaluate the extent of the aortic inflammatory response, and to determine the protective effects of LBP. The levels of TG, TC, MDA and IL‑6 were significantly higher in the mice subjected to chronic composite psychological stress (HS), as compared with the mice treated with LBP alone (HL), or treated with LBP and subjected to stress (HLS). In addition, SOD and HSP‑70 levels, and the mRNA expression levels of CYP7A1 were significantly lower in the HS group, as compared with that in the HL and HLS groups. These results suggest that chronic composite psychological stress may promote the occurrence and development of atherosclerosis, by inducing the aortic inflammatory response and lipid peroxidation. Furthermore, treatment with LBP significantly inhibited oxidative stress and the aortic inflammatory response.