Expression of resistance-nodulation-cell-division efflux pumps in commonly used Burkholderia pseudomallei strains and clinical isolates from northern Australia.
Expression of resistance-nodulation-cell-division efflux pumps in commonly used Burkholderia pseudomallei strains and clinical isolates from northern Australia.
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DOI：
10.1016/s0035-9203(08)70032-4
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发表时间：
2008-12-01
影响因子：
2.2
通讯作者：
Schweizer, Herbert P
中科院分区：
文献类型：
--
作者：
Kumar, Ayush;Mayo, Mark;Schweizer, Herbert P
类鼻疽伯克霍尔德菌是类鼻疽的病原体。由于这种细菌对许多临床有用的抗生素具有内在的抗生素抗性，因此对这种疾病的治疗是漫长的，并且仅限于少数抗生素。B的这些性质。假鼻疽的发生可能部分是由于抵抗-增殖-细胞分裂（RND）家族的外排泵的表达。RND外排泵在B常用菌株和临床分离株中的表达模式和幅度。来自澳大利亚达尔文的皇家达尔文医院的假鼻疽，使用定量实时PCR（qRT-PCR）在生长至晚期指数期的细胞中进行评估。发现三种先前鉴定的RND外排泵AmrAB-OprA、BpeAB-OprB和BpeEF-OprC以及其他四种尚未表征的泵的表达在临床分离株中广泛存在。在50株分离株中的45株（90%）中，检测到7个RND泵中至少一个的mRNA。在这45株分离株中，41株（82%）表达多个泵，其中9株菌株表达所有7个供试泵。虽然这些研究显示RND外排泵表达与临床显著的抗生素耐药性之间没有显着的相关性，但数据支持RND泵可能在这种细菌的生理学，对有毒化合物的防御以及可能的毒力中发挥重要作用的观点。
Burkholderia pseudomallei is the aetiological agent of melioidosis. Therapy for this disease is lengthy and limited to only a few antibiotics because of this bacterium's intrinsic antibiotic resistance to many clinically useful antibiotics. These properties of B. pseudomallei may partially be due to expression of efflux pumps of the resistance-nodulation-cell-division (RND) family. The patterns and magnitude of RND efflux pump expression in commonly used strains and clinical isolates of B. pseudomallei from the Royal Darwin Hospital, Darwin, Australia, were assessed in cells grown to late exponential phase using quantitative real-time PCR (qRT-PCR). Expression of the three previously identified RND efflux pumps AmrAB-OprA, BpeAB-OprB and BpeEF-OprC, as well as four other yet uncharacterized pumps, was found to be widespread in the clinical isolates. In 45 of 50 isolates (90%), mRNA was detected for at least one of the seven RND pumps. Of these 45 isolates, 41 (82%) expressed multiple pumps with nine strains expressing all seven pumps tested. While these studies revealed no striking correlation between RND efflux pump expression and clinically significant antibiotic resistance, the data support the notion that RND pumps probably play important roles in this bacterium's physiology, defence against toxic compounds, and perhaps virulence.