Dppa3 is a marker of pluripotency and has a human homologue that is expressed in germ cell tumours
Dppa3 is a marker of pluripotency and has a human homologue that is expressed in germ cell tumours
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DOI：
10.1159/000074346
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发表时间：
2003-01-01
影响因子：
1.7
通讯作者：
Koopman, P
中科院分区：
文献类型：
--
作者：
Bowles, J;Teasdale, RP;Koopman, P
我们根据其在发育中的小鼠性腺中强烈的雄性特异性表达模式鉴定了一个名为 11M2 的转录本。发现 11M2 在两性的性腺原始生殖细胞 (PGC) 中表达，并且在雌性 PGC 进入第一次减数分裂的前期 I 时下调，与 Oct4 的表达类似。小鼠 EST 分析显示仅在早期胚胎、胚胎干细胞和减数分裂前生殖细胞中表达。 11M2 对应于最近报道的基因，分别称为 PGC7、stella 或 Dppa3。我们鉴定了Dppa3的人类直系同源物，并通过人类EST分析发现它在人类睾丸生殖细胞肿瘤中表达，但在正常人类体组织中不表达。小鼠和人类 DPPA3 在未分化胚胎细胞、胚胎生殖细胞和成体生殖细胞肿瘤中的表达模式共同表明该基因在维持细胞多能性中的作用。版权所有 (C) 2003 S. Karger AG，巴塞尔。
We identified a transcript named 11M2 on the basis of its strong male-specific expression pattern in the developing mouse gonad. 11M2 was found to be expressed by gonad primordial germ cells (PGCs) of both sexes and down-regulated in female PGCs as they enter prophase I of the first meiotic division, similar to the expression of Oct4. Mouse EST analysis revealed expression only in early-stage embryos, embryonic stem cells and pre-meiotic germ cells. 11M2 corresponds to a recently reported gene variously known as PGC7, stella or Dppa3. We have identified the human orthologue of Dppa3 and find by human EST analysis that it is expressed in human testicular germ cell tumours but not in normal human somatic tissues. The expression patterns of mouse and human DPPA3, in undifferentiated embryonic cells, embryonic germ cells and adult germ cell tumours, together suggest a role for this gene in maintaining cell pluripotentiality. Copyright (C) 2003 S. Karger AG, Basel.