Alkaline phosphatase activity in gingival crevicular fluid during canine retraction.
Alkaline phosphatase activity in gingival crevicular fluid during canine retraction.
复制标题
DOI：
10.1111/j.1601-6343.2006.00358.x
复制
发表时间：
2006-02-01
影响因子：
3.1
通讯作者：
Parkash, H
中科院分区：
文献类型：
--
作者：
Batra, P;Kharbanda, Op;Parkash, H
目的：研究人类正畸牙移动过程中牙周沟液中碱性磷酸酶的活性。选择10名需要拔除第一前磨牙的女性患者，接受标准方丝弓机械疗法。实验变量：尖牙后退使用100g前牙合金弹簧。结果：实验开始前(基线)、开始后即刻、第1、7、14、21天，实验侧和对照侧牙周组织中碱性磷酸酶活性均有显著变化(p<0.05)。酶活性高峰出现在回缩开始的第14天，随后活性显着下降，尤其是在近中面。结论：应用量热测定试剂盒可以成功地估计牙周膜中碱性磷酸酶的活性。酶活性随牙齿移动量的不同而不同。
OBJECTIVES: The aim of the study was to investigate alkaline phosphatase activity in the gingival crevicular fluid (GCF) during orthodontic tooth movement in humans.SETTING AND SAMPLE POPULATION: Postgraduate orthodontic clinic. Ten female patients requiring all first premolar extractions were selected and treated with standard edgewise mechanotherapy.EXPERIMENTAL VARIABLE: Canine retraction was done using 100 g sentalloy springs. Maxillary canine on one side acted as experimental site while the contralateral canine acted as control.OUTCOME MEASURE: Gingival crevicular fluid was collected from mesial and distal of canines before initiation of canine retraction (baseline), immediately after initiation of retraction, and on 1st, 7th, 14th and 21st day and the alkaline phosphatase activity was estimated.RESULTS: The results show significant (p < 0.05) changes in alkaline phosphatase activity on the 7th, 14th and 21st day on both mesial and distal aspects of the compared experimental and control sides. The peak in enzyme activity occurred on the 14th day of initiation of retraction followed by a significant fall in activity especially on the mesial aspect.CONCLUSIONS: The study showed that alkaline phosphatase activity could be successfully estimated in the GCF using calorimetric estimation assay kits. The enzyme activity showed variation according to the amount of tooth movement.