Restricted expression of recombination activating gene (RAG‐1) in mouse lymphoid tissues
Restricted expression of recombination activating gene (RAG‐1) in mouse lymphoid tissues
复制标题
重组激活基因（RAG-1）在小鼠淋巴组织中的限制表达
DOI：
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发表时间：
1992
影响因子：
3.6
通讯作者：
K. Hamatani
中科院分区：
文献类型：
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作者：
A. Yamamoto;M. Atsuta;K. Hamatani
为了确定重组酶活性在小鼠胸腺、脾脏和淋巴结中的分布，我们使用原位杂交方法检测了重组激活基因RAG-1和RAG-2的表达。RAG-1在大多数皮质胸腺细胞中表达，但在大多数髓质胸腺细胞中不表达。尽管RAG-2的杂交信号不如RAG-1的杂交信号强烈，但RAG-2转录物的定位与RAG-1的定位相似。在脾脏中，仅在窦附近的有限细胞中发现RAG-1的表达，并且卵泡内的大多数细胞对RAG-1转录物呈阴性。在裸鼠中，在相同区域检测到RAG-1表达细胞，这表明脾脏中RAG-1的原位杂交信号是由于B细胞来源的细胞。在淋巴结中，仅在髓区发现RAG-1的表达。RAG-2转录本在脾脏和淋巴结（如果有的话）中的表达太微弱，无法确定特异性定位。这些结果表明，大多数皮质胸腺细胞和脾脏中的一些细胞能够分别重排T细胞受体基因和免疫球蛋白基因，但在脾脏和淋巴结的RAG-1表达细胞中不能排除一些其他解释的可能性。
In an attempt to determine the distribution of recombinase activity in the mouse thymus, spleen and lymph nodes, we used the in situ hybridization method to examine the expression of the recombination activating genes RAG‐1 and RAG‐2. Expression of RAG‐1 was found in most cortical thymocytes but not in the majority of medullary thymocytes. Although hybridization signals of RAG‐2 were not as intense as those of RAG‐1, the localization of RAG‐2 transcripts was similar to that of RAG‐1. In the spleen, expression of RAG‐1 was found only in limited cells near the sinus, and the majority of the cells within the follicle were negative for RAG‐1 transcript. In nude mice, RAG‐1‐expressing cells were detected in the same regions, which suggests that in situ hybridization signals of RAG‐1 in the spleen are due to the cells of B cell origin. In the lymph nodes, expression of RAG‐1 was found only in the medullary region. Expression of RAG‐2 transcript in the spleen and the lymph nodes, if any, was too faint to determine the specific localization. These results suggest that most of the cortical thymocytes and some cells in the spleen are capable of rearranging T cell receptor genes and immunoglobulin genes, respectively, but the possibility of some other explanation could not be ruled out in RAG‐1 expressing cells of the spleen and the lymph nodes.
影响因子：
56.9
作者：
OETTINGER, MA;SCHATZ, DG;BALTIMORE, D
通讯作者：
BALTIMORE, D
影响因子：
2.7
作者：
COX, KH;DELEON, DV;ANGERER, RC
通讯作者：
ANGERER, RC
影响因子：
56.9
作者：
TURKA, LA;SCHATZ, DG;THOMPSON, CB
通讯作者：
THOMPSON, CB