Ptychographic modulation engine: a low-cost DIY microscope add-on for coherent super-resolution imaging
Ptychographic modulation engine: a low-cost DIY microscope add-on for coherent super-resolution imaging
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DOI：
10.1088/1361-6463/ab489d
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发表时间：
2019-08
期刊：
影响因子：
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通讯作者：
Z. Bian;Shaowei Jiang;Pengming Song;He Zhang;Pouria Hoveida;K. Hoshino;G. Zheng
中科院分区：
文献类型：
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作者：
Z. Bian;Shaowei Jiang;Pengming Song;He Zhang;Pouria Hoveida;K. Hoshino;G. Zheng
生物细胞和组织的成像通常依赖于荧光标记，其提供具有分子特异性的高对比度。然而，外源性标记剂的使用可能改变生物样本的正常生理学。作为已建立的荧光显微镜的补充，无标记定量相位成像为生物样本提供了客观的形态测量工具，并且没有造影剂引入的可变性。在这里，我们报告了一个简单，低成本的显微镜附加，称为重叠关联调制引擎（PME），用于超分辨率定量相位成像。在这个显微镜附加模块中，我们将漫射器连接到3D打印的保持器上，该保持器可以机械地移动到不同的x-y位置。然后，我们使用两个振动电机引入随机的位置偏移的扩散。在大多数现有的显微镜平台中，附加模块可以放置在物镜透镜和样品之间。由于漫射器调制过程，否则无法访问的高分辨率对象信息现在可以被编码到捕获的图像中。在重叠关联相位恢复过程中，我们联合恢复复杂的物体波前，复杂的漫射体轮廓，和未知的位置偏移的漫射体。我们证明了四倍的分辨率增益超过所采用的2×物镜透镜的衍射极限。我们还测试了我们的体外细胞成像方法，我们能够在捕获数据后调整焦点。所报道的显微镜附加装置为超分辨率定量相位成像提供了一种交钥匙解决方案。它可能会发现在无标记生物成像中的应用，其中既需要大视场又需要高分辨率。
Imaging of biological cells and tissues often relies on fluorescent labels, which offer high contrast with molecular specificity. The use of exogenous labeling agents, however, may alter the normal physiology of the bio-specimens. Complementary to the established fluorescence microscopy, label-free quantitative phase imaging provides an objective morphological measurement tool for bio-specimens and is free of variability introduced by contrast agents. Here, we report a simple and low-cost microscope add-on, termed ptychographic modulation engine (PME), for super-resolution quantitative phase imaging. In this microscope add-on module, we attach a diffuser to a 3D-printed holder that can be mechanically moved to different x-y positions. We then use two vibrational motors to introduce random positional shifts to the diffuser. The add-on module can be placed between the objective lens and the specimen in most existing microscope platforms. Thanks to the diffuser modulation process, the otherwise inaccessible high-resolution object information can now be encoded into the captured images. In the ptychographic phase retrieval process, we jointly recover the complex object wavefront, the complex diffuser profile, and the unknown positional shifts of the diffuser. We demonstrate a four-fold resolution gain over the diffraction limit of the employed 2× objective lens. We also test our approach for in vitro cell imaging, where we are able to adjust the focus after the data has been captured. The reported microscope add-on provides a turnkey solution for super-resolution quantitative phase imaging. It may find applications in label-free bio-imaging where both large field-of-view and high resolution are needed.