TILTING OF THE LIGHT-CHAIN REGION OF MYOSIN DURING STEP LENGTH CHANGES AND ACTIVE FORCE GENERATION IN SKELETAL-MUSCLE
TILTING OF THE LIGHT-CHAIN REGION OF MYOSIN DURING STEP LENGTH CHANGES AND ACTIVE FORCE GENERATION IN SKELETAL-MUSCLE
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DOI：
10.1038/375688a0
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发表时间：
1995-06-22
期刊：
影响因子：
64.8
通讯作者：
GOLDMAN, YE
中科院分区：
文献类型：
--
作者：
IRVING, M;ALLEN, TS;GOLDMAN, YE
FORCE generation and relative sliding between the myosin and actin filaments in muscle are thought to be caused by tilting of the head region of the myosin crossbridges between the filaments(1-3). Structural and spectroscopic experiments have demonstrated segmental flexibility of myosin in muscle(4-6), but have not shown a direct linkage between tilting of the myosin heads and either force generation or filament sliding. Here we use fluorescence polarization to detect changes in the orientation of the light-chain region of the head, the part most likely to tilt(5,7,8), and synchronized head movements by imposing rapid length steps(9-11). We found that the light-chain region of the myosin head tilts both during the imposed filament sliding and during the subsequent quick force recovery that is thought to signal the elementary force-generating event.
