Anti-viral activity induced by culturing lymphocytes with tumor-derived or virus-transformed cells. Enhancement of human natural killer cell activity by interferon and antagonistic inhibition of susceptibility of target cells to lysis.
Anti-viral activity induced by culturing lymphocytes with tumor-derived or virus-transformed cells. Enhancement of human natural killer cell activity by interferon and antagonistic inhibition of susceptibility of target cells to lysis.
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DOI：
10.1084/jem.147.5.1314
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发表时间：
1978-05-01
期刊：
影响因子：
--
通讯作者：
Santoli D
中科院分区：
文献类型：
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作者：
Trinchieri G;Santoli D
用病毒或细胞系在淋巴细胞中诱导的干扰素使人淋巴细胞对靶细胞系的天然细胞毒性增加数倍。细胞分离实验支持干扰素增强自然杀伤细胞活性而不是产生新的效应细胞群体的假设。在产生内源性干扰素的淋巴细胞和细胞系的混合培养物中，干扰素介导细胞毒性的增强，其代表高达观察到的细胞毒性的70-90%。干扰素对靶细胞的作用与对淋巴细胞的作用是拮抗的：用干扰素预处理后，各种细胞对细胞介导的裂解的敏感性降低，并且在某些情况下几乎完全被抑制。干扰素使靶细胞抵抗通过需要RNA和蛋白质合成的细胞内机制起作用的自然杀伤细胞。虽然正常的成纤维细胞受到保护，但病毒感染的细胞和大多数肿瘤细胞通常不受干扰素的保护。干扰素通过刺激非常有效的非特异性细胞毒性细胞并同时保护正常细胞免于溶解，可能使自然杀伤细胞系统成为针对肿瘤和病毒感染细胞的诱导性选择性防御机制。
Interferon, induced in lymphocytes either with viruses or cell lines, increases severalfold the natural cytotoxicity of human lymphocytes on target cell lines. Cell separation experiments support the hypothesis that interferon enhances the activity of natural killer cells rather than generating a new population of effector cells. In mixed culture of lymphocytes and cell lines in which endogenous interferon is produced, interferon mediates an enhancement of cytotoxicity that represents up to 70-90% of the observed cytotoxicity. The effect of interferon on target cells is antagonistic to the effect on the lymphocytes: the susceptibility to cell-mediated lysis of various cells upon pretreatment with interferon is decreased and in some cases almost completely suppressed. Interferon renders target cells resistant to natural killer cells acting by an intracellular mechanism which requires RNA and protein synthesis. While normal fibroblasts are protected, virus-infected cells and most tumor cells usually are not protected by interferon. Interferon by stimulating very efficient nonspecific cytotoxic cells and by protecting at the same time normal cells from lysis, might render the natural killer cell system an inducible selective defense mechanism against tumor and virus-infected cells.