Inhibitory effect of simulated microgravity on differentiating preosteoblasts
Inhibitory effect of simulated microgravity on differentiating preosteoblasts
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模拟微重力对前成骨细胞分化的抑制作用
DOI：
10.1016/j.asr.2012.07.036
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发表时间：
2013-01-01
影响因子：
2.6
通讯作者：
Shang, P.
中科院分区：
文献类型：
--
作者：
Hu, L. F.;Qian, A. R.;Shang, P.
微重力引起的骨丢失部分是由于成熟成骨细胞减少所致。本研究采用随机定位仪模拟微重力环境，研究了微重力对2 T3前成骨细胞分化的急性影响。在正常（1g）条件下培养7天后，将细胞暴露于模拟微重力24小时。结果表明，模拟微重力处理24 h，在不改变细胞形态的情况下，显著降低了碱性磷酸酶（ALP）活性。此外，成骨相关基因，包括Runx 2（Runx 2），osterix，骨钙素（OC），I型胶原（Col I）和骨形态发生蛋白（BMP）的mRNA表达显着下调。此外，总细胞外信号调节激酶（Erk）和磷酸化Erk（p-Erk）的蛋白质印迹分析表明，代表Erk活化状态的p-Erk水平增加。总之，我们的研究结果表明，急性暴露于模拟微重力抑制成骨细胞的分化，通过调节成骨基因的表达和Erk活性。这些发现为微重力和卸载引起的骨丢失提供了新的见解。(C)2012年空间研委会。由爱思唯尔有限公司出版。保留所有权利。
The bone loss induced by microgravity is partly due to the decrease of mature osteoblasts. In the present study, we employed the random positioning machine (RPM) to simulate microgravity and investigated the acute effects of simulated microgravity on the differentiation of 2T3 preosteoblasts. Following 7 days' culture under normal (1 g) condition, cells were exposed to simulated microgravity for 24 h. The results showed that 24 h treatment of simulated microgravity significantly decreased alkaline phosphatase (ALP) activity without changing the cell morphology. In addition, the mRNA expressions of osteogenic genes, including runt-related gene 2 (Runx2), osterix, osteocalcin (OC), type I collagen (Col I) and bone morphogenetic protein (BMP), were dramatically downregulated. Moreover, western blot analysis of total extracellular signal-regulated kinase (Erk) and phosphorylated Erk (p-Erk) indicated that p-Erk level, which represents the Erk activation status, was increased. Taken together, our results suggested that acute exposure to simulated microgravity inhibited osteoblast differentiation through modulating the expression of osteogenic genes and the Erk activity. These findings provide new insight for bone loss due to microgravity and unloading. (C) 2012 COSPAR. Published by Elsevier Ltd. All rights reserved.