Chemical Mutagenesis of an Emissive RNA Alphabet.
Chemical Mutagenesis of an Emissive RNA Alphabet.
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发射RNA字母的化学诱变。
DOI：
10.1021/jacs.5b10420
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发表时间：
2015-11-25
影响因子：
15
通讯作者：
Tor Y
中科院分区：
文献类型：
--
作者：
Rovira AR;Fin A;Tor Y
描述了一种进化的荧光核糖核苷字母表，它包括同象的嘌呤(TZA，TZG)和嘧啶(TZU，TZC)类似物，它们都是由异噻唑[4，3-d]嘧啶作为共同的杂环核心衍生而来的。结构和生化分析表明，核苷，特别是C-核苷嘌呤类似物，是天然核苷的忠实同构和等功能替代物，与硫代[3，4-d]-嘧啶衍生的RNA字母表相比显示出更好的特征。将氮恢复到等同于嘌呤N7的位置会导致“等功能”行为，正如腺苷脱氨酶能够像腺苷一样有效地脱氨TZA所说明的那样。
An evolved fluorescent ribonucleoside alphabet comprising isomorphic purine (tzA, tzG) and pyrimidine (tzU, tzC) analogues, all derived from isothiazolo[4,3-d]pyrimidine as a common heterocyclic core, is described. Structural and biochemical analyses illustrate that the nucleosides, particularly the C-nucleosidic purine analogues, are faithful isomorphic and isofunctional surrogates of their natural counterparts and show improved features when compared to an RNA alphabet derived from thieno[3,4-d]-pyrimidine. The restoration of the nitrogen in a position equivalent to the purines’ N7 leads to “isofunctional” behavior, as illustrated by the ability of adenosine deaminase to deaminate tzA as effectively as adenosine, the native substrate.