Effect of Human Endometrial Stromal Cell-derived Conditioned Medium on Uterine Natural Killer (uNK) Cells' Proliferation and Cytotoxicity
Effect of Human Endometrial Stromal Cell-derived Conditioned Medium on Uterine Natural Killer (uNK) Cells' Proliferation and Cytotoxicity
复制标题
人子宫内膜基质细胞来源的条件培养基对子宫自然杀伤（uNK）细胞增殖和细胞毒性的影响
DOI：
10.1111/j.1600-0897.2010.00955.x
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发表时间：
2011-06-01
影响因子：
3.6
通讯作者：
Huang, Lili
中科院分区：
文献类型：
--
作者：
Chen, Yuezhou;Zhuang, Yaling;Huang, Lili
子宫内膜间质细胞参与免疫细胞增殖、凋亡、分化和功能的调节。在子宫内膜中，uNK细胞与基质细胞密切接触。本研究的目的是探讨人子宫内膜间质细胞对uNK细胞增殖和uNK细胞毒活性的影响。研究方法条件培养基来源于增殖期、分泌期和妊娠早期的子宫内膜间质细胞。采用MTS染色和流式细胞术检测基质细胞条件培养液对NK细胞增殖和细胞毒活性的影响。结果分泌期和妊娠早期基质细胞条件培养液均能显著促进NK细胞增殖。与对照组相比，增殖期、分泌期和蜕膜期条件培养液对uNK细胞的杀伤活性均显著降低，但不同生理阶段的抑制能力无显著性差异。结论人子宫内膜间质细胞可能通过影响uNK细胞的增殖和杀伤活性参与其功能的调节。
ProblemHuman endometrial stromal cells are involved in the regulation of immune cell proliferation, apoptosis, differentiation, and function. In the endometrium, uNK cells are in close contact with stromal cells. The aim of the study was to investigate the effects of human endometrial stromal cells on uNK-cell proliferation and uNK-cell cytotoxicity.Method of studyThe conditioned medium was derived from the endometrial stromal cells in the proliferative phase, secretory phase, and early pregnancy. The effects of stromal cell-derived conditioned medium on uNK-cell proliferation and cytotoxicity were detected by mitochondrial lactate dehydrogenase-based MTS staining and flow cytometry.ResultsThe stromal cell-derived conditioned medium in both secretory phase and early pregnancy significantly promoted uNK-cell proliferation. Compared with the control group, the uNK-cell cytotoxicity were significantly reduced by conditioned medium in the proliferative, secretory, and decidua groups, but there were no significant differences among these different physiological stages in the inhibiting ability.ConclusionHuman endometrial stromal cells may be involved in the regulation of uNK-cell functions through influencing proliferation and cytolytic activity.