Differential virulence of Xanthomonas campestris pv. pruni to peach, plum, and apricot cultivars.
Differential virulence of Xanthomonas campestris pv. pruni to peach, plum, and apricot cultivars.
复制标题
野油菜黄单胞菌 pv 的毒力差异。
DOI：
10.1094/phyto-78-1312
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
H. J. Plessis
中科院分区：
文献类型：
--
作者：
H. J. Plessis
材料和方法稳定1.野油菜黄单胞菌致病变种菌株的起源。李子用于细菌菌株和接种物。15株X. C.使用李测试差异毒力（表1）。将冻干的培养物在27 ℃下在Difco营养琼脂（NA）上复苏，并每月在NA斜面上传代培养。在菌株宿主位置分离NA上，从48 h培养物制备接种物混悬液年份。将一环的细菌生长物悬浮在无菌蒸馏的Xcpl Peach（Elberta）Ceres，South Africa 1984水（SDW）中，并调节至λ 6 20 nm = 0.027，对应于Xcp 2 Plum（Golden King）Stellenbosch，South Africa 1984每毫升约5.6 X 10'菌落形成单位（cfu）作为Xcp 3 Peach（圣佩德罗）Elsenburg，南非1985，通过稀释平板法在NA上测定。将Xcp 4 Plum（圣罗莎）Ceres，南非1984在SDW中进一步稀释混悬液，使其含有约1 × 106或5× 104 cfu/ml，并在1小时内使用Xcp 5 Apricot（Supergold）马尔梅斯伯里，南非1985。Xcp 9 Plum New Zealand 1953 Xcp 58 Peach Zimbabwe 1979植物材料。杏（皇家），桃（Bokkeveld，Xcp 62）的品种杏（Blenril）北美
MATERIALS AND METHODSTABLE 1. Origin of strains of Xanthomonas campestris pv. pruni used for Bacterial strains and inoculum. Fifteen strains of X. c. pruni testing differential virulence were used (Table 1). Lyophilized cultures were revived on Difco nutrient agar (NA) at 27 C and subcultured monthly on NA slants. Year of Inoculum suspensions were prepared from 48-hr-old cultures on Strain Host Location isolation NA. A loopful of bacterial growth was suspended in sterile distilled Xcpl Peach (Elberta) Ceres, South Africa 1984 water (SDW) and adjusted to A 6 2 0nm= 0.027 corresponding to Xcp2 Plum (Golden King) Stellenbosch, South Africa 1984 approximately 5.6 X 10'colony-forming units (cfu) per milliliter as Xcp3 Peach (San Pedro) Elsenburg, South Africa 1985 determined by dilution plating on NA. Suspensions were diluted Xcp4 Plum (Santa Rosa) Ceres, South Africa 1984 further in SDW to contain about 1X 106 or 5× 104 cfu/ml and used Xcp5 Apricot (Supergold) Malmesbury, South Africa 1985 within 1 hr. Xcp9 Plum New Zealand 1953 Xcp58 Peach Zimbabwe 1979 Plant material. Cultivars of apricot (Royal), peach (Bokkeveld, Xcp62 Apricot (Blenril) North America