The role of sulfur dioxide in the regulation of mitochondrion-related cardiomyocyte apoptosis in rats with isopropylarterenol-induced myocardial injury.
The role of sulfur dioxide in the regulation of mitochondrion-related cardiomyocyte apoptosis in rats with isopropylarterenol-induced myocardial injury.
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二氧化硫对异丙肾上腺素诱导的心肌损伤大鼠线粒体相关心肌细胞凋亡的调节作用
DOI：
10.3390/ijms140510465
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发表时间：
2013-05-21
影响因子：
5.6
通讯作者：
Du J
中科院分区：
文献类型：
--
作者：
Jin H;Liu AD;Holmberg L;Zhao M;Chen S;Yang J;Sun Y;Chen S;Tang C;Du J
研究了二氧化硫(SO2)对异丙肾上腺素(ISO)盐酸盐所致心肌损伤的调节作用及其机制。将Wistar大鼠随机分为4组：对照组、ISO组、ISO+SO2组和单纯SO2组。测定心功能，检测心肌细胞凋亡率。检测大鼠左心组织中bcl2、bax和细胞色素c(Cytc)的表达及caspase-9、caspase-3活性。分析心肌线粒体通透性转换孔(MPTP)和膜电位的开放状态。结果表明，ISO处理的大鼠出现了心功能障碍和心脏损伤。心肌细胞凋亡率增加，bcl-2表达下调，bax表达上调，线粒体膜电位显著降低，MPTP开放，细胞胞浆释放细胞明显增多，caspase-9和caspase-3活性均升高。但给予SO2供体可明显改善心功能，减轻心肌损伤，减少心肌细胞凋亡，上调心肌bcl2，下调bax表达，刺激线粒体膜电位，关闭MPTP，减少细胞周期蛋白释放，降低左心室组织caspase-9、caspase-3活性。因此，SO2减轻心肌损伤与抑制心肌细胞凋亡有关，bcl2/cytc/caspase-9/caspase-3通路可能参与了这一过程。
The authors investigated the regulatory effects of sulfur dioxide (SO2) on myocardial injury induced by isopropylarterenol (ISO) hydrochloride and its mechanisms. Wistar rats were divided into four groups: control group, ISO group, ISO plus SO2 group, and SO2 only group. Cardiac function was measured and cardiomyocyte apoptosis was detected. Bcl-2, bax and cytochrome c (cytc) expressions, and caspase-9 and caspase-3 activities in the left ventricular tissues were examined in the rats. The opening status of myocardial mitochondrial permeability transition pore (MPTP) and membrane potential were analyzed. The results showed that ISO-treated rats developed heart dysfunction and cardiac injury. Furthermore, cardiomyocyte apoptosis in the left ventricular tissues was augmented, left ventricular tissue bcl-2 expression was down-regulated, bax expression was up-regulated, mitochondrial membrane potential was significantly reduced, MPTP opened, cytc release from mitochondrion into cytoplasm was significantly increased, and both caspase-9 and caspase-3 activities were increased. Administration of an SO2 donor, however, markedly improved heart function and relieved myocardial injury of the ISO-treated rats; it lessened cardiomyocyte apoptosis, up-regulated myocardial bcl-2, down-regulated bax expression, stimulated mitochondrial membrane potential, closed MPTP, and reduced cytc release as well as caspase-9 and caspase-3 activities in the left ventricular tissue. Hence, SO2 attenuated myocardial injury in association with the inhibition of apoptosis in myocardial tissues, and the bcl-2/cytc/caspase-9/caspase-3 pathway was possibly involved in this process.
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影响因子：
4.8
作者：
Boulares, AH;Yakovlev, AG;Smulson, M
通讯作者：
Smulson, M
慢性硫氢化钠治疗可减少自发性高血压大鼠心肌内冠状动脉内侧增厚、间质纤维化和 ROS 产生
DOI：
10.1152/ajpheart.00088.2007
发表时间：
2007-10-01
影响因子：
4.8
作者：
Shi, Ying-Xian;Chen, Ying;Zhu, Yi-Chun
通讯作者：
Zhu, Yi-Chun
DOI：
10.1083/jcb.119.3.493
发表时间：
1992-11
期刊：
The Journal of cell biology
影响因子：
--
作者：
Gavrieli Y;Sherman Y;Ben-Sasson SA
通讯作者：
Ben-Sasson SA
DOI：
10.1186/1471-2210-5-10
发表时间：
2005-04-06
期刊：
BMC pharmacology
影响因子：
--
作者：
Hassan MA;Ketat AF
通讯作者：
Ketat AF
影响因子：
5
作者：
Rich, DQ;Schwartz, J;Dockery, DW
通讯作者：
Dockery, DW