Pax7 identifies neural crest, chromatophore lineages and pigment stem cells during zebrafish development
Pax7 identifies neural crest, chromatophore lineages and pigment stem cells during zebrafish development
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DOI：
10.1387/ijdb.062217al
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发表时间：
2007-01-01
影响因子：
0.7
通讯作者：
Dominguez, Luis
中科院分区：
文献类型：
--
作者：
Lacosta, Ana Ma;Canudas, Jesus;Dominguez, Luis
在早期斑马鱼胚胎发育过程中使用免疫染色，我们报告说，颅和躯干神经嵴表达配对盒蛋白Pax 7，从而揭示了一种新的神经嵴标记在斑马鱼。在头部，我们表明，Pax 7广泛表达在颅嵴细胞，这表明复制的旁系同源组Pax 3/7在脊椎动物的起源包括保守的表达Pax 7在头部神经嵴的所有脊椎动物物种的研究。在躯干中，Pax 7识别迁移前和迁移神经嵴细胞。值得注意的是，我们观察到Pax 7的表达过程中的黑素细胞，xanthophore和虹膜前体细胞的发展。与鸟类中黑素细胞前体的情况相反，Pax 7与早期黑素色素表现出重叠表达。最后，在幼虫到成人的过渡，我们表明，色素干细胞重演的Pax 7的表达。
Using immunostaining during early zebrafish embryogenesis, we report that the cranial and trunk neural crest expresses the paired box protein Pax7, thus revealing a novel neural crest marker in zebrafish. In the head, we show that Pax7 is broadly expressed in the cranial crest cells, which indicates that duplication of the paralogous group Pax3/7 at the origin of vertebrates included the conserved expression of Pax7 in the head neural crest of all of the vertebrates species studied so far. In the trunk, Pax7 recognizes both premigratory and migratory neural crest cells. Notably, we observed the expression of Pax7 during the development of melanophore,xanthophore and iridophore precursor cells. In contrast to the case of melanocyte precursors in birds, Pax7 showed overlapping expression with early melanin pigment. Finally, during the larva to adult transition, we show that pigment stem cells recapitulate the expression of Pax7.