High-efficient and sustainable biodegradation of microcystin-LR using Sphingopyxis sp. YF1 immobilized Fe3O4@chitosan
High-efficient and sustainable biodegradation of microcystin-LR using Sphingopyxis sp. YF1 immobilized Fe3O4@chitosan
复制标题
使用Sphingopyxis sp. 高效且可持续地生物降解微囊藻毒素-LR。
DOI：
10.1016/j.colsurfb.2019.110633
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Yang Fei
中科院分区：
文献类型：
--
作者：
Wu Pian;Li Guiyin;He Yafei;Luo Dan;Li Lei;Guo Jian;Ding Ping;Yang Fei
The microcystin-LR (MC-LR) produced due to harmful cyanobacterial blooms have brought great harm to human and aquatic organisms, attracting a wide public health attention. To deal with MC-LR contamination, we synthesized a novel bio-functionalized composite for the high-efficient and sustainable biodegradation of microcystin-LR by covalent immobilizingSphingopyxissp. YF1 onto chitosan-grafted Fe3O4magnetic particles (Fe3O4@CTS). The Fourier transform infrared spectroscopy (FTIR), Field emission scanning electron microscopy (SEM) and vibrating sample magnetometry (VSM) were utilized to characterize the structural properties of Fe3O4@CTS/Sphingopyxis sp. YF1. The immobilization conditions were optimized. And the MC-LR-degrading capabilities of Fe3O4@CTS/Sphingopyxissp. YF1 were assessed under various conditions. The results showed that the optimal immobilization conditions containing 1.0 % (v/v) glutaraldehyde, immobilization for 4 h at 30 ℃. The Fe3O4@CTS/Sphingopyxissp. YF1 showed an attractive degradation performance which possesed a wide torlerance to pH (6.0–9.0) and temperature (25–35 ℃). More interesting is that the Fe3O4@CTS/Sphingopyxissp. YF1 exhibited significantly increased MC-LR-degrading capabilities after recycling and reusing which degradation rate reached 1.50 μg/mL/h in the sixth cycle, and it was easily recycled by using a magnet (Ms 21.5 emug-1). Two intermediates (tetrapeptide and Adda) and three degradation related genes (mlrA,mlrB andmlrC) were obtained in this study and the pathway for the degradation was proposed. These results revealed that Fe3O4@CTS/Sphingopyxissp. YF1 can be potentially used for treatment of MC-LR contaminated environment.
